| Field | Specification |
|---|---|
| Target | |
| Alternative names | Alpha-enolase; 2-phospho-D-glycerate hydro-lyase; C-myc promoter-binding protein; Enolase 1; MBP-1; MPB-1; Non-neural enolase; NNE; Phosphopyruvate hydratase; Plasminogen-binding protein; ENO1; ENO1L1; MBPB1; MPB1 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human ENO1 recombinant protein (Position: R50-E250). |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets alpha-enolase (gene ENO1) in human samples and is validated for Western blot, IHC, flow cytometry and ELISA.
Alpha-enolase (ENO1) is the glycolytic enzyme that interconverts 2-phosphoglycerate and phosphoenolpyruvate, and it is expressed in most tissues. It can also appear on the cell surface as a plasminogen-binding receptor, and an alternative product (MBP-1) represses the MYC promoter, so ENO1 is studied in metabolism, cancer and autoimmunity.
| Target | Alpha-enolase (gene ENO1; UniProt P06733, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human |
| Purification | Immunogen affinity purified |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Reconstitution | 500 µg/mL after adding 0.2 mL distilled water |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human) |
|---|---|
| IHC (paraffin sections) | 2–5 µg/mL (Human) |
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
| ELISA | 0.1–0.5 µg/mL |
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Immunogen
Recombinant human ENO1 fragment (Arg50–Glu250), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human. UniProt places alpha-enolase in the cytoplasm and cell membrane. Tissue expression noted by UniProt (human): The alpha/alpha homodimer is expressed in embryo and in most adult tissues.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Guanghui Li et al. (2019) Piwi-Interacting RNA1037 Enhances Chemoresistance and Motility in Human Oral Squamous Cell Carcinoma Cells. OncoTargets and Therapy. 10.2147/OTT.S233322
