| Field | Specification |
|---|---|
| Target | |
| Alternative names | Ectonucleotide pyrophosphatase/phosphodiesterase family member 5; E-NPP 5; NPP-5; ENPP5 |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human ENPP5 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 27E97 is a rabbit monoclonal antibody that recognizes ectonucleotide pyrophosphatase/phosphodiesterase family member 5 (gene ENPP5) in human, mouse and rat samples and is validated for Western blot and ICC/IF. The predicted molecular weight is 54.7 kDa, and the supplier reports an observed band at about 55 kDa.
ENPP5 is a member of the ectonucleotide pyrophosphatase/phosphodiesterase family. It can hydrolyze NAD but not nucleotide di- or triphosphates and lacks lysophospholipase D activity; it may take part in neuronal cell communication, but its physiological role is not yet well defined.
| Target | Ectonucleotide pyrophosphatase/phosphodiesterase family member 5 (gene ENPP5; UniProt Q9UJA9, human) |
|---|---|
| Clone | 27E97 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 54.7 kDa |
| Observed MW | 55 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
Samples with a confirmed band (WB): MCF-7 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human ENPP5.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt describes ectonucleotide pyrophosphatase/phosphodiesterase family member 5 as secreted and also located in the membrane. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
