| Field | Specification |
|---|---|
| Target | |
| Alternative names | Nucleoside diphosphate phosphatase ENTPD5; CD39 antigen-like 4; ER-UDPase; Ectonucleoside triphosphate diphosphohydrolase 5; NTPDase 5; Guanosine-diphosphatase ENTPD5; GDPase ENTPD5; Inosine diphosphate phosphatase ENTPD5; Nucleoside diphosphatase; Uridine-diphosphatase ENTPD5; UDPase ENTPD5; ENTPD5; CD39L4; PCPH |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human ENTPD5 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 21E75 is a rabbit monoclonal antibody that recognizes nucleoside diphosphate phosphatase ENTPD5 in human, mouse and rat samples and is validated for Western blot. The predicted molecular weight is 47.5 kDa, and the supplier reports an observed band at about 48 kDa.
ENTPD5 is an endoplasmic reticulum nucleoside diphosphatase that prefers UDP and GDP. By converting UDP to UMP it supports the calnexin/calreticulin glycoprotein folding cycle and N-glycosylation, and it has been linked to increased glycolysis downstream of PI3K-AKT signaling in growing cells.
| Target | Nucleoside diphosphate phosphatase ENTPD5 (gene ENTPD5; UniProt O75356, human) |
|---|---|
| Clone | 21E75 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 47.5 kDa |
| Observed MW | 48 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|
Samples with a confirmed band (WB): LnCaP cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human ENTPD5.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt describes nucleoside diphosphate phosphatase ENTPD5 as secreted and also located in the endoplasmic reticulum. Tissue expression noted by UniProt (human): Expressed in adult liver, kidney, prostate, testis and colon. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
