| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | C-C motif chemokine 26;CC chemokine IMAC;Eotaxin-3;Macrophage inflammatory protein 4-alpha;MIP-4-alpha;Small-inducible cytokine A26;Thymic stroma chemokine-1;TSC-1;CCL26;SCYA26;UNQ216/PRO242; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of human Eotaxin 3. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
Anti-Eotaxin 3/CCL26 Antibody Picoband® is an antibody targeting CCL26. Common applications include WB, IHC, Flow Cytometry, ELISA. Key specifications include host: Rabbit; clonality: Polyclonal; isotype: Rabbit IgG; reactivity: Human; observed MW: 11 kDa; calculated MW: 10648 MW.
Boster Bio Anti-Eotaxin 3/CCL26 Antibody catalog # PA1451. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Key elements and design rationale
- Target: CCL26 — C-C motif chemokine 26
- Antibody format: Host: Rabbit; Clonality: Polyclonal; Isotype: Rabbit IgG
- Species reactivity: Human
- Molecular weight guidance: Observed: 11 kDa; Calculated: 10648 MW
Specificity note: No cross reactivity with other proteins.
Biological background
Protein function (datasheet): Chemotactic for eosinophils and basophils. Binds to CCR3.
Scientific background (datasheet): Eotaxin-3 is a small cytokine belonging to the CC chemokine family that is also called CCL26, Chemokine (C-C motif) ligand 26. Chemokines play pivotal roles in health and disease by controlling migration and tissue localization of specific types of cells expressing their cognate receptors. Eotaxin-3 is chemotactic for eosinophils and basophils and elicits its effects by binding to the cell surface chemokine receptor CCR3. It is expressed by several tissues including heart, lung and ovary, and in endothelial cells that have been stimulated with the cytokine interleukin 4. This gene for chemokine is located on human chromosome 7. Eotaxin-3 induced migration of eosinophils and basophils only at a 10-fold higher concentration than eotaxin.
Cellular localization (datasheet): Secreted.
Tissue details (datasheet): Ubiquitously expressed at low levels in various tissues including heart and ovary.
Sequence similarities (datasheet): Belongs to the intercrine beta (chemokine CC) family.
Research relevance and current trends
- Commonly studied in contexts related to Chemokines,Immunology,Innate Immunity.
- Supports comparative expression analysis across conditions, genotypes, or treatments when paired with appropriate controls.
- Useful for confirming target presence and subcellular distribution using orthogonal readouts (e.g., microscopy vs. immunoblotting).
Common research applications
- Western blot (WB): Compare relative target abundance and apparent size/isoforms across samples; interpret bands in light of expected MW and potential PTMs.
- ELISA: Measure target abundance in compatible matrices using a standard-curve readout; ensure dilution linearity and appropriate controls.
- Immunohistochemistry (IHC): Assess tissue distribution and cell-type patterns; interpret staining with appropriate negative controls and antigen context.
- Flow cytometry: Quantify target-positive populations in single-cell suspensions; pair with viability and isotype/FMO controls conceptually.
Notes for experimental interpretation
- Consider isoforms, post-translational modifications, and processing that can shift apparent molecular weight or localization.
- Cross-reactivity (datasheet): No cross-reactivity with other proteins
- Use appropriate positive and negative controls (e.g., KO/KD, blocking peptide, or isotype controls) to support specificity interpretation.
As a polyclonal antibody, this reagent may recognize multiple epitopes on the target, which can improve detection robustness but may require careful specificity controls.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
