| Field | Specification |
|---|---|
| Target | |
| Alternative names | Eotaxin;C-C motif chemokine 11;Eosinophil chemotactic protein;Small-inducible cytokine A11;Ccl11;Scya11; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of mouse Eotaxin, identical to the related rat sequence. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects eotaxin (gene Ccl11) in mouse and rat samples and is validated for Western blot. The predicted molecular weight is 10.9 kDa, and the supplier reports an observed band at about 37 kDa.
Eotaxin (CCL11) is a C-C chemokine that recruits eosinophils and other leukocytes, mainly by binding the receptor CCR3. It is a key mediator of eosinophil accumulation in allergic inflammation, including asthma and atopic disease, and it has also been studied as a factor whose circulating levels rise with age.
| Target | Eotaxin (gene Ccl11; UniProt P48298, mouse) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg thimerosal; 0.05 mg NaN3; plus stabilizers |
| Calculated MW | 10.9 kDa |
| Observed MW | 37 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Mouse, Rat) |
|---|
Samples with a confirmed band (WB): recombinant mouse Eotaxin protein.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide from the C-terminal region of mouse eotaxin. The peptide sequence is conserved in rat.
Reactivity Notes
The supplier lists reactivity with mouse and rat. UniProt describes eotaxin as a secreted protein. Tissue expression noted by UniProt (mouse): Expressed constitutively in the thymus. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
