| Field | Specification |
|---|---|
| Target | |
| Alternative names | Rap guanine nucleotide exchange factor 4; Exchange factor directly activated by cAMP 2; Exchange protein directly activated by cAMP 2; EPAC 2; cAMP-regulated guanine nucleotide exchange factor II; cAMP-GEFII; RAPGEF4; CGEF2; EPAC2 |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human Epac2 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 22R17 is a rabbit monoclonal antibody that recognizes rap guanine nucleotide exchange factor 4 (gene RAPGEF4) in human, mouse and rat samples and is validated for Western blot and IHC. The predicted molecular weight is 115.5 kDa, and the supplier reports an observed band at about 116 kDa.
EPAC2 (RAPGEF4) is a cAMP-activated exchange factor for Rap1 and Rap2 that is abundant in the brain and endocrine tissues. It mediates PKA-independent effects of cAMP, including potentiation of insulin granule exocytosis in pancreatic β-cells, and it is studied in secretion and neuronal plasticity.
| Target | Rap guanine nucleotide exchange factor 4 (gene RAPGEF4; UniProt Q8WZA2, human) |
|---|---|
| Clone | 22R17 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 115.5 kDa |
| Observed MW | 116 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
Samples with a confirmed band (WB): human fetal brain.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Immunogen
Synthetic peptide based on the sequence of human epac2.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places rap guanine nucleotide exchange factor 4 in the cytoplasm and membrane. Tissue expression noted by UniProt (human): Predominantly expressed in brain and adrenal gland. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
