| Field | Specification |
|---|---|
| Target | |
| Alternative names | Epithelial cell adhesion molecule;Ep-CAM;Adenocarcinoma-associated antigen;Cell surface glycoprotein Trop-1;Epithelial cell surface antigen;Epithelial glycoprotein;EGP;Epithelial glycoprotein 314;EGP314;hEGP314;KS 1/4 antigen;KSA;Major gastrointestinal tumor-associated protein GA733-2;Tumor-associated calcium signal transducer 1;CD326;EPCAM;GA733-2, M1S2, M4S1, MIC18, TACSTD1, TROP1; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human EpCAM |
| Molecular weight | |
| Purification | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone CIB-5 is a rabbit monoclonal antibody that recognizes epithelial cell adhesion molecule (gene EPCAM) in human, mouse and rat samples and is validated for Western blot, IHC and ICC/IF. The predicted molecular weight is 34.9 kDa, and the supplier reports an observed band at about 35–40 kDa.
EpCAM (CD326) is a transmembrane glycoprotein on most epithelial cells that mediates homophilic cell-cell adhesion. It also influences proliferation and stem cell maintenance, and it is highly expressed in many carcinomas. EpCAM is widely used to identify and isolate epithelial and circulating tumor cells, and EPCAM mutations cause congenital tufting enteropathy.
| Target | Epithelial cell adhesion molecule (gene EPCAM; UniProt P16422, human) |
|---|---|
| Clone | CIB-5 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 34.9 kDa |
| Observed MW | 35–40 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
Samples with a confirmed band (WB): human Caco-2 cells, human MCF-7 cells, rat small intestine tissue, mouse small intestine tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:500 overnight at 4 °C, HRP-conjugated secondary antibody at 1:500, ECL detection.
Samples with confirmed staining (IHC): mouse small intestine tissue, rat small intestine tissue, human breast tissue, human colon tissue, human colon cancer tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Immunogen
Synthetic peptide based on the sequence of human epCAM.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places epithelial cell adhesion molecule in the lateral cell membrane and cell junction. Tissue expression noted by UniProt (human): Highly and selectively expressed by undifferentiated rather than differentiated embryonic stem cells (ESC). The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Pi Zhao et al. (2026) A self-amplifying cuproptosis nanomedicine to overcome immunosuppression by blocking tumor-derived exosomes for enhancing lung cancer immunotherapy. Journal of Controlled Release. 10.1016/j.jconrel.2025.114593
