| Field | Specification |
|---|---|
| Target | |
| Alternative names | Ephrin type-A receptor 5;2.7.10.1;Brain-specific kinase;EPH homology kinase 1;EHK-1;EPH-like kinase 7;EK7;hEK7;EPHA5;BSK, EHK1, HEK7, TYRO4; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of human Eph receptor A5, different from the related mouse sequence by two amino acids. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects ephrin type-A receptor 5 (gene EPHA5) in human, mouse and rat samples and is validated for Western blot. The predicted molecular weight is 114.8 kDa, and the supplier reports an observed band at about 114 kDa.
EphA5 is an Eph-family receptor tyrosine kinase that binds GPI-anchored ephrin-A ligands. It is expressed mainly in the nervous system, where Eph-ephrin signaling guides axons, sets up topographic maps and influences synapse formation.
| Target | Ephrin type-A receptor 5 (gene EPHA5; UniProt P54756, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3 |
| Calculated MW | 114.8 kDa |
| Observed MW | 114 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Mouse, Rat, Human) |
|---|
Samples with a confirmed band (WB): rat brain tissue, mouse brain tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Synthetic peptide from the C-terminal region of human eph receptor A5. It differs from the mouse sequence at two residues.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places ephrin type-A receptor 5 in the cell membrane and cell projection. Tissue expression noted by UniProt (human): Almost exclusively expressed in the nervous system in cortical neurons, cerebellar Purkinje cells and pyramidal neurons within the cortex and hippocampus. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
