| Field | Specification |
|---|---|
| Target | |
| Alternative names | Bifunctional epoxide hydrolase 2; EPHX2 |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human EPHX2 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 29E21 is a rabbit monoclonal antibody that recognizes bifunctional epoxide hydrolase 2 (gene EPHX2) in human samples and is validated for Western blot. The predicted molecular weight is 62.6 kDa, and the supplier reports an observed band at about 63 kDa.
Soluble epoxide hydrolase (EPHX2) is a bifunctional enzyme with a C-terminal epoxide hydrolase domain and an N-terminal lipid phosphatase domain. It converts epoxyeicosatrienoic acids and other lipid epoxides into less active diols, thereby regulating vascular tone and inflammation, and it is a studied target in cardiovascular and pain research.
| Target | Bifunctional epoxide hydrolase 2 (gene EPHX2; UniProt P34913, human) |
|---|---|
| Clone | 29E21 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 62.6 kDa |
| Observed MW | 63 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|
Samples with a confirmed band (WB): MCF-7 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human EPHX2.
Reactivity Notes
The supplier lists reactivity with human. UniProt places bifunctional epoxide hydrolase 2 in the cytoplasm and peroxisome. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
