| Field | Specification |
|---|---|
| Target | |
| Alternative names | Erythropoietin receptor; EPO-R; Epor |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E. coli-derived mouse EPO Receptor recombinant protein (Position: D32-E225). |
| Molecular weight | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects erythropoietin receptor (gene Epor) in mouse and rat samples and is validated for ELISA and Western blot. The predicted molecular weight is 55.2 kDa, and the supplier reports an observed band at about 55 kDa.
The erythropoietin receptor (EPOR) is a type I cytokine receptor found mainly on erythroid progenitor cells. Binding of EPO rearranges the receptor dimer and activates JAK2, which drives STAT5 and other pathways that promote red blood cell production. EPOR is studied in erythropoiesis and hematologic disorders.
| Target | Erythropoietin receptor (gene Epor; UniProt P14753, mouse) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3 |
| Calculated MW | 55.2 kDa |
| Observed MW | 55 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL |
|---|---|
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): rat lung tissue, human U87-MG cells, human A431 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 50 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:10000, ECL detection.
Immunogen
Recombinant mouse EPO Receptor fragment (Asp32–Glu225), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with mouse and rat. UniProt places erythropoietin receptor in the cell membrane and SUBCELLULAR LOCATION:. Tissue expression noted by UniProt (mouse): Expressed in relatively mature erythroid progenitor cells and in EPO-responsive erythroleukemia cells. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
