| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Epidermal growth factor receptor substrate 15; Protein Eps15; Protein AF-1p; EPS15; AF1P |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | E.coli-derived human EPS15 recombinant protein (Position: M1-K295). |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
Anti-EPS15 Antibody Picoband® is an antibody for EPS15 detection raised in Rabbit (Polyclonal, Rabbit IgG), with reported reactivity: Human,Mouse,Rat. Commonly used in WB, IHC, Flow Cytometry, ELISA workflows.
Key elements and design rationale
- Target: EPS15 (epidermal growth factor receptor pathway substrate 15); UniProt: P42566
- Antibody format: Rabbit, Polyclonal, Rabbit IgG
- Molecular weight: 138 kDa, calculated 50514 MW
- Applications: WB, IHC, Flow Cytometry, ELISA
Vendor description (summary): Boster Bio Anti-EPS15 Antibody Picoband® catalog # A01681-1.
Biological background
Biological context: Involved in cell growth regulation. May be involved in the regulation of mitogenic signals and control of cell proliferation. Involved in the internalization of ligand-inducible receptors of the receptor tyrosine kinase (RTK) type, in particular EGFR. Plays a role in the assembly of clathrin-coated pits (CCPs). Acts as a clathrin adapter required for post-Golgi trafficking. Seems to be involved in CCPs maturation including invagination or budding. Involved in endocytosis of integrin beta-1 (ITGB1) and transferrin receptor (TFR); internalization of ITGB1 as DAB2-dependent cargo but not TFR seems to require association with DAB2.
Expression and localization notes: cellular localization: Cell membrane. Peripheral membrane protein. Cytoplasmic side. Cytoplasm. Clathrin-coated pit., tissue context: Ubiquitously expressed..
Common research applications
- Western blotting (WB): Compare EPS15 levels across samples and conditions using appropriate loading and biological controls.
- Immunohistochemistry (IHC): Evaluate spatial distribution of EPS15 in tissue sections, considering fixation and antigen retrieval effects.
- Flow cytometry: Quantify EPS15-positive populations in single-cell suspensions with appropriate gating and controls.
- ELISA: Use antibody-based detection formats to assess antigen presence or binding in plate-based assays.
Notes for experimental interpretation
- Account for isoforms, post-translational modifications, and sample-specific processing that can shift apparent molecular weight or epitope accessibility.
- Use positive/negative biological controls where possible (e.g., known-expressing cells/tissues, knockdown/knockout models) and include appropriate secondary-only/isotype controls for imaging workflows.
Additional product notes (from provided fields)
- Specificity: No cross reactivity with other proteins.
- Background: Epidermal growth factor receptor substrate 15 is a protein that in humans is encoded by the EPS15 gene. It is mapped to 1p32.3. This gene encodes a protein that is part of the EGFR pathway. The protein is present at clatherin-coated pits and is involved in receptor-mediated endocytosis of EGF. Notably, this gene is rearranged with the HRX/ALL/MLL gene in acute myelogeneous leukemias. Alternative splicing results in multiple transcript variants encoding distinct isoforms.
- Cross reactivity: No cross-reactivity with other proteins.
- Cellular localization: Cell membrane. Peripheral membrane protein. Cytoplasmic side. Cytoplasm. Clathrin-coated pit.
- Tissue details: Ubiquitously expressed.
- Research category: Adapters,Signal Transduction,Transmembrane
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
