| Field | Specification |
|---|---|
| Target | |
| Alternative names | Epsin-1; EH domain-binding mitotic phosphoprotein; EPS-15-interacting protein 1; EPN1 |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human Epsin 1 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 28E39 is a rabbit monoclonal antibody that recognizes epsin-1 (gene EPN1) in human, mouse and rat samples and is validated for Western blot and ICC/IF. The predicted molecular weight is 60.3 kDa, and the supplier reports an observed band at about 90 kDa.
Epsin-1 (EPN1) is an endocytic adaptor that binds PI(4,5)P2-rich membranes through its ENTH domain, bending the membrane to help form clathrin-coated pits. It also binds ubiquitinated cargo, clathrin and AP-2, and supports receptor-mediated endocytosis of signaling receptors.
| Target | Epsin-1 (gene EPN1; UniProt Q9Y6I3, human) |
|---|---|
| Clone | 28E39 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 60.3 kDa |
| Observed MW | 90 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
Samples with a confirmed band (WB): K562 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human epsin 1.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places epsin-1 in the cytoplasm, cell membrane, nucleus and membrane. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
