| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Calretinin; CR; 29 kDa calbindin; CALB2; CAB29 |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | E.coli-derived human ERAP2 recombinant protein (Position: K13-T960). |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
Anti-ERAP2 Antibody Picoband® is an antibody for ERAP2 detection raised in Rabbit (Polyclonal, Rabbit IgG), with reported reactivity: Human. Commonly used in WB, IHC, Flow Cytometry, ELISA workflows.
Key elements and design rationale
- Target: ERAP2 (calbindin 2); UniProt: Q6P179
- Antibody format: Rabbit, Polyclonal, Rabbit IgG
- Molecular weight: 110 kDa
- Applications: WB, IHC, Flow Cytometry, ELISA
Vendor description (summary): Boster Bio Anti-ERAP2 Antibody Picoband® catalog # A04269-1.
Biological background
Biological context: Calretinin is a calcium-binding protein which is abundant in auditory neurons.
Expression and localization notes: cellular localization: Cuticular plate. Cytosol. Nucleus. Synaptic membrane. Dendrite. Gap junction. Neuron projection. Parallel fiber to Purkinje cell synapse. Presynapse. Stereocilium. Synapse., tissue context: Brain..
Common research applications
- Western blotting (WB): Compare ERAP2 levels across samples and conditions using appropriate loading and biological controls.
- Immunohistochemistry (IHC): Evaluate spatial distribution of ERAP2 in tissue sections, considering fixation and antigen retrieval effects.
- Flow cytometry: Quantify ERAP2-positive populations in single-cell suspensions with appropriate gating and controls.
- ELISA: Use antibody-based detection formats to assess antigen presence or binding in plate-based assays.
Notes for experimental interpretation
- Account for isoforms, post-translational modifications, and sample-specific processing that can shift apparent molecular weight or epitope accessibility.
- Use positive/negative biological controls where possible (e.g., known-expressing cells/tissues, knockdown/knockout models) and include appropriate secondary-only/isotype controls for imaging workflows.
Additional product notes (from provided fields)
- Background: Endoplasmic reticulum aminopeptidase 2 is a protein that in humans is encoded by the ERAP2 gene. This gene encodes a zinc metalloaminopeptidase of the M1 protease family that resides in the endoplasmic reticulum and functions in N-terminal trimming antigenic epitopes for presentation by major histocompatibility complex (MHC) class I molecules. Certain mutations in this gene are associated with the inflammatory arthritis syndrome ankylosing spondylitis and pre-eclampsia. This gene is located adjacent to a closely related aminopeptidase gene on chromosome 5.
- Cross reactivity: No cross-reactivity with other proteins.
- Cellular localization: Cuticular plate. Cytosol. Nucleus. Synaptic membrane. Dendrite. Gap junction. Neuron projection. Parallel fiber to Purkinje cell synapse. Presynapse. Stereocilium. Synapse.
- Tissue details: Brain.
- Research category: Adaptive Immunity,B Cells,Hematopoietic Progenitors,Immunoglobulins,Immunology,Lymphoid,Receptors,Stem Cells
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
