| Field | Specification |
|---|---|
| Target | |
| Alternative names | Receptor tyrosine-protein kinase erbB-3;2.7.10.1;Proto-oncogene-like protein c-ErbB-3;Tyrosine kinase-type cell surface receptor HER3;ERBB3;HER3; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human ErbB (HER3) |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Overview
This product is an anti-ERBB3 antibody for target detection and characterization. Key identifiers include host species: Rabbit; Monoclonal; clone BIB-5; isotype Rabbit IgG; reactivity: Human,Rat. Reported application contexts include WB, IP (as provided in the source record). Boster Bio Anti-ErbB (HER3) Rabbit Monoclonal Antibody catalog # M00539. Tested in WB, IP applications. This antibody reacts with Human, Rat.
Key elements and design rationale
- Target: ERBB3 (Receptor tyrosine-protein kinase erbB-3).
- Antibody format: Monoclonal; clone BIB-5; isotype Rabbit IgG.
- Host: Rabbit.
- Species reactivity: Human,Rat (confirm in your model system with appropriate controls).
This description is intended to help interpret the antibody design and the biological context of the target using the fields provided in the catalog record, alongside general experimental considerations.
Biological background
ERBB3 (protein: T-cell surface glycoprotein CD4) is a commonly studied target in molecular and cellular biology. Functional context (as provided): Binds and is activated by neuregulins and NTAK. May also be activated by CSPG5. . Reported cellular localization context: Isoform 1: Cell membrane; Single-pass type I membrane protein. Tissue expression notes (as provided): Epithelial tissues and brain.
Research relevance and current trends
- Research context keywords from the source record include: Apoptosis,Cell Biology,Developmental Biology,Growth Factors/Hormones,Intracellular,Kinases,Nervous System Development,Organogenesis,Protein Phosphorylation,Receptor Tyrosine Kinases,Signal Transduction,Tyrosine Kinases.
- Current studies often focus on connecting target abundance/localization to pathway perturbations across models, tissues, and cell states.
- Quantitative and multiplexed assays (e.g., imaging + immunoblot panels) are commonly used to compare phenotypes across conditions and time-courses.
Common research applications
- Western blotting (WB): assess relative target abundance across samples, treatments, or time-points.
- Immunoprecipitation (IP): enrich target complexes for downstream immunoblot or interaction analyses.
Workflow ideas (metafield): Validate ERBB3 antibody specificity using KO/KD control samples (WB/IF/IHC as appropriate), Detect ERBB3 expression by Western blot in cell or tissue lysates, Enrich ERBB3 by immunoprecipitation from lysates for downstream analysis
Notes for experimental interpretation
- Consider isoforms and post-translational modifications (PTMs) that may shift apparent molecular weight or epitope accessibility.
- Apparent molecular weight may vary by sample type and processing (observed MW: 54 kDa; calculated MW: 148098 MW).
- Control concepts: include appropriate negative controls (e.g., isotype, KO/KD samples) and orthogonal validation when feasible.
Additional product details (from the source record)
- Molecular weight (observed): 54 kDa
- Cellular localization (provided): Isoform 1: Cell membrane; Single-pass type I membrane protein.
- Tissue details (provided): Epithelial tissues and brain.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
