| Field | Specification |
|---|---|
| Target | |
| Alternative names | Receptor tyrosine-protein kinase erbB-4;2.7.10.1;Proto-oncogene-like protein c-ErbB-4;Tyrosine kinase-type cell surface receptor HER4;p180erbB4;ERBB4 intracellular domain;4ICD;E4ICD;s80HER4;ERBB4;HER4; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human ErbB (HER4) |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone HC-5 is a rabbit monoclonal antibody that recognizes receptor tyrosine-protein kinase erbB-4 (gene ERBB4) in human, mouse and rat samples and is validated for Western blot, ICC/IF, flow cytometry and immunoprecipitation. The predicted molecular weight is 146.8 kDa, and the supplier reports an observed band at about 180 kDa.
HER4 (ERBB4) is an EGFR-family receptor tyrosine kinase activated by neuregulins and some EGF-family ligands. It is needed for normal development of the heart, nervous system and mammary gland, and its cleaved intracellular domain can move to the nucleus to regulate gene expression.
| Target | Receptor tyrosine-protein kinase erbB-4 (gene ERBB4; UniProt Q15303, human) |
|---|---|
| Clone | HC-5 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 146.8 kDa |
| Observed MW | 180 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Immunoprecipitation | 1:20 |
| Flow cytometry | 1:20 |
Samples with a confirmed band (WB): human T-47D cells, human MCF-7 cells, human HeLa cells, mouse brain tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 8% SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1: 500 overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (ICC/IF): A673 cells.
Immunogen
Synthetic peptide based on the sequence of human erbB (HER4).
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places receptor tyrosine-protein kinase erbB-4 in the cell membrane. Tissue expression noted by UniProt (human): Expressed at highest levels in brain, heart, kidney, in addition to skeletal muscle, parathyroid, cerebellum, pituitary, spleen, testis and breast. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
