| Field | Specification |
|---|---|
| Target | |
| Alternative names | DNA excision repair protein ERCC-8; Cockayne syndrome WD repeat protein CSA; ERCC8; CKN1; CSA |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human ERCC8 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 26E15 is a rabbit monoclonal antibody that recognizes DNA excision repair protein ERCC-8 (gene ERCC8) in human samples and is validated for Western blot and immunoprecipitation. The predicted molecular weight is 44.1 kDa, and the supplier reports an observed band at about 44 kDa.
CSA (ERCC8) is a WD40-repeat protein that serves as the substrate receptor of a CUL4-DDB1 E3 ubiquitin ligase. It acts in transcription-coupled nucleotide excision repair, which clears lesions that block RNA polymerase II, and mutations in ERCC8 cause Cockayne syndrome type A.
| Target | DNA excision repair protein ERCC-8 (gene ERCC8; UniProt Q13216, human) |
|---|---|
| Clone | 26E15 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 44.1 kDa |
| Observed MW | 44 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunoprecipitation | 1:50 |
Samples with a confirmed band (WB): Molt4 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human ERCC8.
Reactivity Notes
The supplier lists reactivity with human. UniProt places DNA excision repair protein ERCC-8 in the nucleus, chromosome and nucleus matrix. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
