| Field | Specification |
|---|---|
| Target | |
| Alternative names | Mitogen-activated protein kinase 3;MAP kinase 3;MAPK 3;2.7.11.24;ERT2;Extracellular signal-regulated kinase 1;ERK-1;Insulin-stimulated MAP2 kinase;MAP kinase isoform p44;p44-MAPK;Microtubule-associated protein 2 kinase;p44-ERK1;MAPK3;ERK1, PRKM3; |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human ERK1/2 |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone DFF-13 is a rabbit monoclonal antibody that recognizes MAPK3 in human, mouse and rat samples and is validated for Western blot, ICC/IF, immunoprecipitation and flow cytometry.
| Target | MAPK3 (UniProt P27361/P28482) |
|---|---|
| Clone | DFF-13 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Observed MW | 41, 43 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Immunoprecipitation | 1:20 |
| Flow cytometry | 1:20 |
Samples with a confirmed band (WB): human HeLa cells, human HepG2 cells, human MCF-7 cells, rat PC-12 cells, rat C6 cells, mouse NIH3T3 cells, mouse Neuro-2a whole cellICC/IF: HeLa cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 10% SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:500 overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (ICC/IF): mouse pancreas, mouse brain, human prostate cancer, rat brain, rat colon, rat kidney, human breast cancer, human colon cancer, human liver cancer, human pancreatic cancer.
Immunogen
Synthetic peptide based on the sequence of human ERK1/2.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
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Shuting Li et al. (2024) Ursolic acid, an inhibitor of TMEM16A, co-loaded with cisplatin in hydrogel drug delivery system for multi-targeted therapy of lung cancer. International Journal of Biological Macromolecules. 10.1016/j.ijbiomac.2024.134587
Xiang Li et al. (2023) Transcriptome, network analysis, and molecular docking approaches to elucidate the modes of action of Astragaloside IV against myocardial infarction with mitochondrial energy metabolism network-targeted regulation. Computers in Biology and Medicine. 10.1016/j.compbiomed.2023.107599
Xiaoyan Wei et al. (2023) mPFC DUSP1 mediates adolescent cocaine exposure-induced higher sensitivity to drug in adulthood. EMBO Reports. 10.15252/embr.202356981
Li Sha-sha et al. (2025) Therapeutic potential of voltage-dependent potassium channel subtype 1.3 blockade in alleviating macrophage-related renal inflammation and fibrogenesis. Cell Death Discovery. 10.1038/s41420-025-02508-7
Zheng Yanyan et al. (2023) Paternal methamphetamine exposure induces higher sensitivity to methamphetamine in male offspring through driving ADRB1 on CaMKII-positive neurons in mPFC. Translational Psychiatry. 10.1038/s41398-023-02624-x
Mengguo Han et al. (2022) Ameliorative effects of epigallocatechin-3-gallate nanoparticles on 2,4-dinitrochlorobenzene induced atopic dermatitis: A potential mechanism of inflammation-related necroptosis. Frontiers in Nutrition. 10.3389/fnut.2022.953646
Xue Bai et al. (2022) Nuciferine Inhibits TMEM16A in Dietary Adjuvant Therapy for Lung Cancer. Journal of Agricultural and Food Chemistry. 10.1021/acs.jafc.1c08375
Yanfei He et al. (2023) Structural characteristics and immunomodulation activity of a polysaccharide from purslane (Portulaca oleracea). Journal of Functional Foods. 10.1016/j.jff.2023.105781
