| Field | Specification |
|---|---|
| Target | |
| Alternative names | Mitogen-activated protein kinase 7;MAP kinase 7;MAPK 7;2.7.11.24;Big MAP kinase 1;BMK-1;Extracellular signal-regulated kinase 5;ERK-5;MAPK7;BMK1, ERK5, PRKM7; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human ERK5 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone HCD-13 is a rabbit monoclonal antibody that recognizes mitogen-activated protein kinase 7 (gene MAPK7) in human, mouse and rat samples and is validated for Western blot, ICC/IF, immunoprecipitation and flow cytometry. The predicted molecular weight is 88.4 kDa, and the supplier reports an observed band at about 115 kDa.
ERK5 (MAPK7), also called big MAP kinase 1, is activated specifically by MEK5 in response to growth factors and cellular stress. Once active it moves to the nucleus and phosphorylates targets such as MEF2 transcription factors, and it is important for cardiovascular development, endothelial function and cell survival.
| Target | Mitogen-activated protein kinase 7 (gene MAPK7; UniProt Q13164, human) |
|---|---|
| Clone | HCD-13 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 88.4 kDa |
| Observed MW | 115 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Immunoprecipitation | 1:20 |
| Flow cytometry | 1:20 |
Immunogen
Synthetic peptide based on the sequence of human ERK5.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places mitogen-activated protein kinase 7 in the cytoplasm and nucleus. Tissue expression noted by UniProt (human): Expressed in many adult tissues.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
