| Field | Specification |
|---|---|
| Target | |
| Alternative names | Thioredoxin domain-containing protein 12; Endoplasmic reticulum resident protein 18; ER protein 18; ERp18; Endoplasmic reticulum resident protein 19; ER protein 19; ERp19; Thioredoxin-like protein p19; hTLP19; TXNDC12; TLP19 |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human ERp19 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 27T78 is a rabbit monoclonal antibody that recognizes thioredoxin domain-containing protein 12 (gene TXNDC12) in human, mouse and rat samples and is validated for Western blot, IHC and ICC/IF. The predicted molecular weight is 19.2 kDa, and the supplier reports an observed band at about 17 kDa.
TXNDC12 (also called ERp18 or ERp19) is a small thioredoxin-like protein of the endoplasmic reticulum. It takes part in the thiol–disulfide exchange reactions that form disulfide bonds in newly made proteins, and is studied in ER protein folding and ER stress.
| Target | Thioredoxin domain-containing protein 12 (gene TXNDC12; UniProt O95881, human) |
|---|---|
| Clone | 27T78 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 19.2 kDa |
| Observed MW | 17 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
Samples with a confirmed band (WB): human 293T cells, human THP-1 cells, human HepG2 cells, human U251 cells, rat liver tissue, mouse liver tissue, mouse pancreas tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:500 overnight at 4 °C, HRP-conjugated secondary antibody at 1:500, ECL detection.
Immunogen
Synthetic peptide based on the sequence of human ERp19.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places thioredoxin domain-containing protein 12 in the endoplasmic reticulum lumen. Tissue expression noted by UniProt (human): Widely expressed. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
