| Field | Specification |
|---|---|
| Target | |
| Alternative names | Estrogen-related receptor gamma; ERR gamma-2; Estrogen receptor-related protein 3; Nuclear receptor subfamily 3 group B member 3; ESRRG; ERR3; ERRG2; KIAA0832; NR3B3 |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human ESRRG |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 25E06 is a rabbit monoclonal antibody that recognizes estrogen-related receptor gamma (gene ESRRG) in human, mouse and rat samples and is validated for Western blot. The predicted molecular weight is 51.3 kDa, and the supplier reports an observed band at about 51 kDa.
Estrogen-related receptor gamma (ESRRG, ERRγ) is an orphan nuclear receptor that activates transcription without a bound ligand. It is expressed in energy-demanding tissues such as heart, muscle, kidney and brain, where it supports mitochondrial and oxidative metabolism genes.
| Target | Estrogen-related receptor gamma (gene ESRRG; UniProt P62508, human) |
|---|---|
| Clone | 25E06 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 51.3 kDa |
| Observed MW | 51 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|
Samples with a confirmed band (WB): rat heart tissue, mouse heart tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 10% SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:500 overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Synthetic peptide based on the sequence of human ESRRG.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places estrogen-related receptor gamma in the nucleus. Tissue expression noted by UniProt (human): Expressed in the heart, kidney, brain, lung, bone marrow, adrenal gland, trachea, spinal cord and thyroid gland. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
