| Field | Specification |
|---|---|
| Target | |
| Alternative names | Protein C-ets-1; p54; ETS1; EWSR2 |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human ETS1 Transcription factor. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone ADAO-5 is a rabbit monoclonal antibody that recognizes protein C-ets-1 (gene ETS1) in human and mouse samples and is validated for Western blot and flow cytometry. The predicted molecular weight is 50.4 kDa, and the supplier reports an observed band at about 46 kDa.
ETS1 is the founding member of the ETS family of transcription factors. It regulates genes involved in lymphocyte development and function, including cytokine and chemokine genes, as well as endothelial cell migration and angiogenesis. ETS1 is studied in immune regulation, vascular biology and tumor invasion.
| Target | Protein C-ets-1 (gene ETS1; UniProt P14921, human) |
|---|---|
| Clone | ADAO-5 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 50.4 kDa |
| Observed MW | 46 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Flow cytometry | 1:20 |
Samples with a confirmed band (WB): human Jurkat cells, human HEL cells, human RT4 cells, human U251 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 10% SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:500 overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Synthetic peptide based on the sequence of human ETS1.
Reactivity Notes
The supplier lists reactivity with human and mouse. UniProt places protein C-ets-1 in the nucleus and cytoplasm. Tissue expression noted by UniProt (human): Highly expressed within lymphoid cells. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
