| Field | Specification |
|---|---|
| Target | |
| Alternative names | Transcription factor ETV6; ETS translocation variant 6; ETS-related protein Tel1; Tel; ETV6; TEL1 |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human ETV6 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 25E79 is a rabbit monoclonal antibody that recognizes transcription factor ETV6 in human samples and is validated for Western blot, ICC/IF and flow cytometry. The predicted molecular weight is 53.0 kDa, and the supplier reports an observed band at about 53 kDa.
ETV6 (also called TEL) is an ETS-family transcriptional repressor that is important for hematopoiesis and for blood stem cell survival. It is frequently involved in chromosomal translocations in leukemia, for example the ETV6-RUNX1 fusion, and inherited ETV6 variants cause thrombocytopenia with predisposition to leukemia.
| Target | Transcription factor ETV6 (gene ETV6; UniProt P41212, human) |
|---|---|
| Clone | 25E79 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 53.0 kDa |
| Observed MW | 53 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Flow cytometry | 1:50 |
Samples with a confirmed band (WB): A431 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human ETV6.
Reactivity Notes
The supplier lists reactivity with human. UniProt places transcription factor ETV6 in the nucleus. Tissue expression noted by UniProt (human): Ubiquitous. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
