| Field | Specification |
|---|---|
| Target | |
| Alternative names | Fatty acid-binding protein, liver;Fatty acid-binding protein 1;Liver-type fatty acid-binding protein;L-FABP;FABP1;FABPL; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human FABP1 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone AACB-6 is a rabbit monoclonal antibody that recognizes fatty acid-binding protein, liver (gene FABP1) in human, mouse and rat samples and is validated for Western blot and flow cytometry. The predicted molecular weight is 14.2 kDa, and the supplier reports an observed band at about 14 kDa.
Liver fatty acid-binding protein (FABP1, L-FABP) is an abundant cytosolic protein in hepatocytes and enterocytes. It binds long-chain fatty acids, acyl-CoAs, bilirubin and other hydrophobic molecules, helping move them within the cell for metabolism, and it is studied in hepatic lipid handling and as a marker of liver and kidney injury.
| Target | Fatty acid-binding protein, liver (gene FABP1; UniProt P07148, human) |
|---|---|
| Clone | AACB-6 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 14.2 kDa |
| Observed MW | 14 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Flow cytometry | 1:20 |
Samples with a confirmed band (WB): human HCCT cells, human HCCP cells, human HepG2 cells, rat liver tissue, human RH35 cells, mouse liver tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:500 overnight at 4 °C, HRP-conjugated secondary antibody at 1:500, ECL detection.
Immunogen
Synthetic peptide based on the sequence of human FABP1.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places fatty acid-binding protein, liver in the cytoplasm. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
