| Field | Specification |
|---|---|
| Target | |
| Alternative names | Fatty acid-binding protein, adipocyte;Adipocyte lipid-binding protein;ALBP;Adipocyte-type fatty acid-binding protein;A-FABP;AFABP;Fatty acid-binding protein 4;FABP4; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence at the N-terminus of human FABP4, identical to the related mouse and rat sequences. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets fatty acid-binding protein, adipocyte (gene FABP4) in human, mouse and rat samples and is validated for IHC and Western blot. The predicted molecular weight is 14.7 kDa, and the supplier reports an observed band at about 15 kDa.
FABP4 (also called aP2 or A-FABP) is a cytoplasmic fatty acid-binding protein expressed mainly in adipocytes and macrophages. It shuttles long-chain fatty acids inside the cell, and circulating FABP4 has been studied as a marker linked to insulin resistance and cardiovascular risk.
| Target | Fatty acid-binding protein, adipocyte (gene FABP4; UniProt P15090, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3; plus stabilizers |
| Calculated MW | 14.7 kDa |
| Observed MW | 15 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| IHC (paraffin sections) | 0.5–1 µg/mL (Mouse, Rat, Human) |
|---|---|
| Western blot | 0.1–0.5 µg/mL (Human, Mouse, Rat) |
Samples with a confirmed band (WB): human RT4 cells, human U251 cells, rat heart tissue, mouse heart tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): mouse intestine tissue, rat intestine tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Immunogen
Synthetic peptide from the N-terminal region of human FABP4. The peptide sequence is conserved in mouse and rat.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places fatty acid-binding protein, adipocyte in the cytoplasm and nucleus. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Chen Rui et al. (2025) Endothelial HMGB1-AIM2 axis worsens myocardial ischemia—reperfusion injury by regulating endothelial pyroptosis. Apoptosis. 10.1007/s10495-025-02162-5
Gaoyang Chen et al. (2020) Circular RNA CDR1as promotes adipogenic and suppresses osteogenic differentiation of BMSCs in steroid-induced osteonecrosis of the femoral head. Bone. 10.1016/j.bone.2020.115258
Feifei Lin et al. (2025) LncRNA H19 promotes adipogenic differentiation disorder by sponging miR-130b-3p to upregulate PPARγ in steroid-induced osteonecrosis of the femoral head. Frontiers in Genetics. 10.3389/fgene.2025.1529797
Jing Wang et al. (2026) The TNF-α–FABP4–Dhhc7 Axis Mediates Olanzapine-Induced Insulin Resistance in Rats and Humans. Clinical and Experimental Pharmacology and Physiology. 10.1111/1440-1681.70104
