| Field | Specification |
|---|---|
| Target | |
| Alternative names | Long-chain-fatty-acid--CoA ligase 4; Arachidonate--CoA ligase; Long-chain acyl-CoA synthetase 4; LACS 4; ACSL4; ACS4; FACL4; LACS4 |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from FACL4 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 26A48 is a rabbit monoclonal antibody that recognizes long-chain-fatty-acid--CoA ligase 4 (gene ACSL4) in human, mouse and rat samples and is validated for Western blot, IHC, ICC/IF, immunoprecipitation and flow cytometry. The predicted molecular weight is 79.2 kDa, and the supplier reports an observed band at about 79 kDa.
Long-chain acyl-CoA synthetase 4 (ACSL4) prefers polyunsaturated fatty acids such as arachidonic acid and channels them into membrane phospholipids. Because these lipids are the main targets of lipid peroxidation, ACSL4 is a key determinant of ferroptosis and is widely used as a ferroptosis marker.
| Target | Long-chain-fatty-acid--CoA ligase 4 (gene ACSL4; UniProt O60488, human) |
|---|---|
| Clone | 26A48 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 79.2 kDa |
| Observed MW | 79 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Immunoprecipitation | 1:50 |
| Flow cytometry | 1:50 |
Samples with a confirmed band (WB): human HeLa cells, human 293T cells, human HepG2 cells, human HEL cells, rat lung tissue, mouse lung tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:500 overnight at 4 °C, HRP-conjugated secondary antibody at 1:500, ECL detection.
Immunogen
Synthetic peptide based on the sequence of FACL4.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places long-chain-fatty-acid--CoA ligase 4 in the mitochondrion outer membrane, endoplasmic reticulum membrane and cell membrane. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Kaiqiang Sun et al. (2025) Glycolysis-Derived Lactate Induces ACSL4 Expression and Lactylation to Activate Ferroptosis during Intervertebral Disc Degeneration. Advanced Science. 10.1002/advs.202416149
Juan Sheng et al. (2026) Lycorine Suppresses DPP3-Mediated Nrf2 Signaling, Thereby Reducing SLC7A11 Levels to Trigger Ferroptosis in Colorectal Cancer Cells. Phytomedicine. 10.1016/j.phymed.2026.158221
Renjie Qiu et al. (2025) Diselenide Nanogels Modulate Mitochondrial Function and Mitigate Oxidative Stress in Cardiomyocytes for Enhanced Cardiac Repair. ACS Applied Materials & Interfaces. 10.1021/acsami.4c22685
Xin Zhou et al. (2023) Transcriptomics and Molecular Docking Reveal the Potential Mechanism of Lycorine Against Pancreatic Cancer. Phytomedicine. 10.1016/j.phymed.2023.155128
Caiwang Peng et al. (2024) Quercetin inhibits hydrogen peroxide-induced cleavage of heat shock protein 90 to prevent glutathione peroxidase 4 degradation via chaperone-mediated autophagy. Phytomedicine. 10.1016/j.phymed.2024.156286
Chuan-zhi Zhao et al. (2024) Exploring the mechanism of Ling-Gui-Zhu-Gan decoction in metabolic cardiomyopathy via inhibiting ferroptosis. Phytomedicine. 10.1016/j.phymed.2024.156234
Xiang Zheng et al. (2024) Delay the progression of glucocorticoid-induced osteoporosis: Fraxin targets ferroptosis via the Nrf2/GPX4 pathway. Phytotherapy Research. 10.1002/ptr.8310
Lingfeng Lai et al. (2026) SPI1 promotes endothelial cell ferroptosis and intracranial aneurysm progression via EP300-mediated FOSL2 acetylation. Journal of Cerebral Blood Flow and Metabolism. 10.1177/0271678X261463947
Wang Zhiqin et al. (2026) Ischemic Stroke Induces Ferroptosis and Neuroinflammation via Activation of the Microglial Tbp-Lpl Transcriptional Axis. Neurochemical Research. 10.1007/s11064-026-04812-9
Zhi-qiang Song et al. (2026) Clinical significance of immunocyte- and ferroptosis-related markers for the prognosis of patients with tenosynovial giant cell tumor: A cross-sectional study. Cancer Treatment and Research Communications. 10.1016/j.ctarc.2026.101269
