| Field | Specification |
|---|---|
| Target | |
| Alternative names | Coagulation factor IX; Christmas factor; Plasma thromboplastin component; PTC; F9 |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human Factor IX |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 18F05 is a rabbit monoclonal antibody that recognizes coagulation factor IX (gene F9) in human samples and is validated for Western blot. The predicted molecular weight is 51.8 kDa, and the supplier reports an observed band at about 52 kDa.
Coagulation factor IX (F9) is a vitamin K-dependent serine protease of the intrinsic pathway. Activated factor IXa, together with factor VIIIa, calcium and phospholipid, converts factor X to factor Xa. F9 mutations cause hemophilia B, and factor IX has been a leading model for gene therapy research.
| Target | Coagulation factor IX (gene F9; UniProt P00740, human) |
|---|---|
| Clone | 18F05 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 51.8 kDa |
| Observed MW | 52 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|
Samples with a confirmed band (WB): human plasma.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human factor IX.
Reactivity Notes
The supplier lists reactivity with human. UniProt describes coagulation factor IX as a secreted protein. Tissue expression noted by UniProt (human): Detected in blood plasma (at protein level). The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Afnan Deebani et al. (2025) Knockdown of tmem183a in Zebrafish Causes Thrombocytopenia and Reduces Coagulation Factors, Disrupting Hemostasis. Thrombosis and Haemostasis. 10.1055/a-2713-0711
