| Field | Specification |
|---|---|
| Target | |
| Alternative names | FAS-associated death domain protein; FAS-associating death domain-containing protein; Mediator of receptor induced toxicity; Fadd; Mort1 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of mouse FADD, which shares 63.6% and 81.8% amino acid (aa) sequence identity with human and rat FADD, respectively. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects FAS-associated death domain protein (gene Fadd) in mouse and rat samples and is validated for Western blot, IHC and flow cytometry. The predicted molecular weight is 23.0 kDa, and the supplier reports an observed band at about 30 kDa.
FADD is an adaptor protein with a death domain and a death effector domain. It is recruited to activated death receptors such as FAS and TNFR1, where it brings in caspase-8 or caspase-10 to form the death-inducing signaling complex (DISC). FADD is essential for extrinsic apoptosis and also regulates necroptosis and immune signaling.
| Target | FAS-associated death domain protein (gene Fadd; UniProt Q61160, mouse) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.005 mg NaN3 |
| Calculated MW | 23.0 kDa |
| Observed MW | 30 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.25 µg/mL (Mouse, Rat) |
|---|---|
| IHC (paraffin sections) | 2–5 µg/mL (Mouse) |
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Mouse) |
Samples with a confirmed band (WB): mouse brain tissue, mouse stomach tissue, mouse lung tissue, mouse liver tissue, mouse kidney tissue, mouse spleen tissue, mouse thymus tissue, mouse NIH/3T3 cells, rat brain tissue, rat stomach tissue, rat lung tissue, rat liver tissue, rat kidney tissue, rat spleen tissue, rat thymus tissue, rat PC-12 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.25 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): mouse liver tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Samples with a confirmed signal (flow cytometry): HEPA1–6 cells.
Immunogen
Synthetic peptide from the C-terminal region of mouse FADD. Sequence identity with the human and rat orthologs: 63.6% and 81.8%, respectively.
Reactivity Notes
The supplier lists reactivity with mouse and rat. UniProt places FAS-associated death domain protein in the cytoplasm. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
