| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Hemoglobin subunit alpha;Alpha-globin;Hemoglobin alpha chain;HBA1;HBA2; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of mouse FADD, which shares 63.6% and 81.8% amino acid (aa) sequence identity with human and rat FADD, respectively. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
Anti-FADD Antibody Picoband® is an antibody for Fadd detection raised in Rabbit (Polyclonal, Rabbit IgG), with reported reactivity: Mouse,Rat. Commonly used in WB, IHC, Flow Cytometry, ELISA workflows.
Key elements and design rationale
- Target: Fadd (Hemoglobin subunit alpha); UniProt: Q61160
- Antibody format: Rabbit, Polyclonal, Rabbit IgG
- Molecular weight: 30 kDa, calculated 15258 MW
- Applications: WB, IHC, Flow Cytometry, ELISA
Vendor description (summary): Boster Bio Anti-FADD Antibody Picoband® catalog # A00237-3.
Biological background
Biological context: Involved in oxygen transport from the lung to the various peripheral tissues.
Expression and localization notes: cellular localization: Secreted., tissue context: Red blood cells..
Common research applications
- Western blotting (WB): Compare Fadd levels across samples and conditions using appropriate loading and biological controls.
- Immunohistochemistry (IHC): Evaluate spatial distribution of Fadd in tissue sections, considering fixation and antigen retrieval effects.
- Flow cytometry: Quantify Fadd-positive populations in single-cell suspensions with appropriate gating and controls.
- ELISA: Use antibody-based detection formats to assess antigen presence or binding in plate-based assays.
Notes for experimental interpretation
- Account for isoforms, post-translational modifications, and sample-specific processing that can shift apparent molecular weight or epitope accessibility.
- Use positive/negative biological controls where possible (e.g., known-expressing cells/tissues, knockdown/knockout models) and include appropriate secondary-only/isotype controls for imaging workflows.
Additional product notes (from provided fields)
- Background: FADD, Fas-Associated protein with Death Domain, is a universal adaptor protein in apoptosis that mediates signaling of all known death domain-containing members of the TNF receptor superfamily. The FADD gene contains 2 exons and spans approximately 3.6 kb. By analysis of somatic cell hybrid panels and by fluorescence in situ hybridization, the FADD gene is mapped to 11q13.3. The protein encoded by this gene is an adaptor molecule that interacts with various cell surface receptors and mediates cell apoptotic signals. Through its C-terminal death domain, this protein can be recruited by TNFRSF6/Fas-receptor, tumor necrosis factor receptor, TNFRSF25, and TNFSF10/TRAIL-receptor, thus, it participates in the death signaling initiated by these receptors.
- Cross reactivity: No cross-reactivity with other proteins
- Cellular localization: Secreted.
- Tissue details: Red blood cells.
- Research category: Adaptive Immunity,Cancer,Cytokines,Immunology,Innate Immunity,Interleukins,Tumor Immunology
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
