| Field | Specification |
|---|---|
| Target | |
| Alternative names | Focal adhesion kinase 1;FADK 1;2.7.10.2;Focal adhesion kinase-related nonkinase;FRNK;Protein phosphatase 1 regulatory subunit 71;PPP1R71;Protein-tyrosine kinase 2;p125FAK;pp125FAK;PTK2;FAK, FAK1; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human FAK |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone DDD-16 is a rabbit monoclonal antibody that recognizes focal adhesion kinase 1 (gene PTK2) in human, mouse and rat samples and is validated for Western blot, IHC, ICC/IF, immunoprecipitation and flow cytometry. The predicted molecular weight is 119.2 kDa, and the supplier reports an observed band at about 119 kDa.
Focal adhesion kinase (FAK, PTK2) is a cytoplasmic tyrosine kinase recruited to focal adhesions after integrin engagement. It relays signals from the extracellular matrix and growth factor receptors to control cell migration, adhesion turnover, proliferation and survival, and it is essential for embryonic development, making it a common target in cancer and cell motility research.
| Target | Focal adhesion kinase 1 (gene PTK2; UniProt Q05397, human) |
|---|---|
| Clone | DDD-16 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 119.2 kDa |
| Observed MW | 119 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Immunoprecipitation | 1:30 |
| Flow cytometry | 1:50 |
Samples with a confirmed band (WB): human HeLa cells, human MCF-7 cells, human HepG2 cells, human A549 cells, rat testis tissue, rat C6 cells, mouse testis tissue, mouse NIH/3T3 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:500 overnight at 4 °C, HRP-conjugated secondary antibody at 1:500, ECL detection.
Samples with confirmed staining (IHC): mouse spleen tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Samples with confirmed staining (ICC/IF): HeLa cells.
Immunogen
Synthetic peptide based on the sequence of human FAK.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places focal adhesion kinase 1 in the cell junction, cell membrane and cytoplasm. Tissue expression noted by UniProt (human): Detected in B and T-lymphocytes. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Weizhen Chen et al. (2018) Multilayered coating of titanium implants promotes coupled osteogenesis and angiogenesis in vitro and in vivo. Acta Biomaterialia. 10.1016/j.actbio.2018.04.043
Gao Ranran et al. (2025) Yijing Decoction improves premature ovarian failure in rats by activating VEGF/VEGFR-2/FAK pathway. Journal of Ovarian Research. 10.1186/s13048-025-01679-2
Binay Sevgi et al. (2022) Transcription factor Runx2 changes the expression of some matricellular proteins in metastatic breast cancer cells. Molecular Biology Reports. 10.1007/s11033-022-07457-3
B-Z Shan et al. (2019) Effect of celecoxib on protein expression of FAK and Cx43 in DMBA induced rat tongue carcinoma cells. European Review for Medical and Pharmacological Sciences. 10.26355/eurrev_201911_19439
Guo‑Qiang Su et al. (2014) Research of shRNAmir inhibitory effects towards focal adhesion kinase expression in the treatment of gastric cancer. Oncology Letters. 10.3892/ol.2014.2725
Ting Cao et al. (2013) Correlation between matrix metalloproteinase expression and activation of the focal adhesion kinase signaling pathway in herpes stromal keratitis. Experimental and Therapeutic Medicine. 10.3892/etm.2013.1407
