| Field | Specification |
|---|---|
| Target | |
| Alternative names | Long-chain fatty acid transport protein 3; FATP-3; Fatty acid transport protein 3; Arachidonate--CoA ligase; Long-chain-fatty-acid--CoA ligase; Solute carrier family 27 member 3; Very long-chain acyl-CoA synthetase homolog 3; VLCS-3; SLC27A3; ACSVL3; FATP3 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human FATP3/SLC27A3 recombinant protein (Position: L192-I683). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets long-chain fatty acid transport protein 3 (gene SLC27A3) in human and mouse samples and is validated for Western blot, IHC and ELISA. The predicted molecular weight is 73.6 kDa, and the supplier reports an observed band at about 70–79 kDa.
FATP3 (SLC27A3) is a member of the fatty acid transport protein family that functions mainly as an acyl-CoA synthetase for long and very long-chain fatty acids. It may also support fatty acid uptake, and it has been studied in lipid metabolism of endothelial and other cell types.
| Target | Long-chain fatty acid transport protein 3 (gene SLC27A3; UniProt Q5K4L6, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 73.6 kDa |
| Observed MW | 70–79 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human, Mouse) |
|---|---|
| IHC (paraffin sections) | 2–5 µg/mL (Human) |
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): human MCF-7 cells, human Hacat cells, mouse NIH/3T3 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): human colorectal adenocarcinoma tissue, human esophageal squamous carcinoma tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Immunogen
Recombinant human FATP3/SLC27A3 fragment (Leu192–Ile683), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human and mouse. UniProt places long-chain fatty acid transport protein 3 in the mitochondrion membrane. Tissue expression noted by UniProt (human): Expressed in bronchial and bronchiolar epithelial cells (at protein level). The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
