| Field | Specification |
|---|---|
| Target | |
| Alternative names | Fatty acid-binding protein 5; Epidermal-type fatty acid-binding protein; E-FABP; Fatty acid-binding protein, epidermal; Keratinocyte lipid-binding protein; Psoriasis-associated fatty acid-binding protein homolog; PA-FABP; Fabp5; Fabpe; Klbp; Mal1 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence at the N-terminus of mouse FABP5, which shares 81.2% amino acid (aa) sequence identity with human FABP5. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects fatty acid-binding protein 5 (gene Fabp5) in human, mouse and rat samples and is validated for ICC/IF and Western blot. The predicted molecular weight is 15.1 kDa, and the supplier reports an observed band at about 15 kDa.
Fatty acid-binding protein 5 (FABP5, also called epidermal FABP) is a cytosolic lipid carrier expressed in skin, brain, immune cells and other tissues. It transports fatty acids and endocannabinoids and can deliver certain fatty acids to the nucleus to activate PPARβ/δ, influencing cell growth and differentiation.
| Target | Fatty acid-binding protein 5 (gene Fabp5; UniProt Q05816, mouse) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 15.1 kDa |
| Observed MW | 15 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL |
|---|---|
| Immunocytochemistry / Immunofluorescence | 5 µg/mL |
Samples with a confirmed band (WB): human RT4 cells, human PC-3 cells, rat lung tissue, mouse lung tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (ICC/IF): A549 cells.
Immunogen
Synthetic peptide from the N-terminal region of mouse FABP5. Sequence identity with the human ortholog: 81.2%.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places fatty acid-binding protein 5 in the cytoplasm, nucleus, synapse and postsynaptic density. Tissue expression noted by UniProt (mouse): Widely expressed. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Qiang Li et al. (2016) High FABP5 Versus CRABPII Expression Ratio in Recurrent Craniopharyngiomas: Implications for Future Treatment. World Neurosurgery. 10.1016/j.wneu.2016.05.063
