| Field | Specification |
|---|---|
| Target | |
| Alternative names | Low affinity immunoglobulin gamma Fc region receptor II-a; IgG Fc receptor II-a; CDw32; Fc-gamma RII-a; Fc-gamma-RIIa; FcRII-a; CD32; FCGR2A; CD32; FCG2; FCGR2A1; IGFR2 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E. coli-derived human FCGR2A recombinant protein (Position: Q34-M210). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes low affinity immunoglobulin gamma Fc region receptor II-a (gene FCGR2A) in human, mouse and rat samples and is validated for ELISA, IHC and Western blot. The predicted molecular weight is 35.0 kDa, and the supplier reports an observed band at about 40 kDa.
FcγRIIA (FCGR2A, CD32A) is a low-affinity activating receptor for the Fc region of IgG found on myeloid cells and platelets. It binds IgG immune complexes and signals through an ITAM motif to promote phagocytosis of opsonized particles and inflammatory responses. It is studied in antibody effector function, platelet activation and autoimmunity.
| Target | Low affinity immunoglobulin gamma Fc region receptor II-a (gene FCGR2A; UniProt P12318, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 35.0 kDa |
| Observed MW | 40 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL |
|---|---|
| IHC (paraffin sections) | 0.5–1 µg/mL |
| ELISA (capture antibody) | 1–5 µg/mL |
Samples with a confirmed band (WB): human THP-1 cells, rat brain tissue, mouse Neuro-2a cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 50 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:10000, ECL detection.
Samples with confirmed staining (IHC): human lung cancer tissue, mouse spleen tissue, rat spleen tissue, human mammary cancer tissue, human placenta tissue, human rectal cancer tissue, human tonsil tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in citrate buffer (pH 6).
Immunogen
Recombinant human FCGR2A fragment (Gln34–Met210), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places low affinity immunoglobulin gamma Fc region receptor II-a in the cell membrane. Tissue expression noted by UniProt (human): Found on monocytes, neutrophils and eosinophil platelets. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Priyanka Khare et al. (2017) Myelin oligodendrocyte glycoprotein-specific antibodies from multiple sclerosis patients exacerbate disease in a humanized mouse model. Journal of Autoimmunity. 10.1016/j.jaut.2017.09.002
Manuela De Michele et al. (2023) SARS-CoV-2 infection predicts larger infarct volume in patients with acute ischemic stroke. Frontiers in Cardiovascular Medicine. 10.3389/fcvm.2022.1097229
Chunyan Li et al. (2026) Intrathecal injection of rituximab inhibits microglial M1 polarisation to alleviate neuropsychiatric SLE symptoms via the cAMP/PKA/CREB signalling pathway. Lupus Science & Medicine. 10.1136/lupus-2025-001774
