| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Low affinity immunoglobulin gamma Fc region receptor II-a; IgG Fc receptor II-a; CDw32; Fc-gamma RII-a; Fc-gamma-RIIa; FcRII-a; CD32; FCGR2A; CD32; FCG2; FCGR2A1; IGFR2 |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | E. coli-derived human FCGR2A recombinant protein (Position: Q34-M210). |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
This antibody is intended for detection of FCGR2A (Keratin, type I cytoskeletal 14) in biological samples using common immunoassay formats. It is typically selected based on target identity, species reactivity, clonality/clone information, and detection modality.
Vendor notes: Boster Bio Anti-FCGR2A Antibody Picoband® catalog # A01450-1. Tested in ELISA, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Key elements and design rationale
- Antibody format: Rabbit Polyclonal Rabbit IgG
- Immunogen / epitope context: E. coli-derived human FCGR2A recombinant protein (Position: Q34-M210). (reported region: Q34-M210).
- Molecular weight context: reported MW: 40 kDa; calculated MW: 51561 MW
- Reactivity: Human,Mouse,Rat
- Applications: ELISA, IHC, WB
As a polyclonal antibody, the reagent recognizes multiple epitopes on the target, which can improve detection robustness but may increase sensitivity to sample-dependent epitope changes.
Biological background
Keratin, type I cytoskeletal 14; Fc fragment of IgG receptor IIa. Low affinity immunoglobulin gamma Fc region receptor II-a is a protein that in humans is encoded by the FCGR2A gene. This gene encodes one member of a family of immunoglobulin Fc receptor genes found on the surface of many immune response cells. It is mapped to 1q23.3. The protein encoded by this gene is a cell surface receptor found on phagocytic cells such as macrophages and neutrophils, and is involved in the process of phagocytosis and clearing of immune complexes. Alternative splicing results in multiple transcript variants. Functional note: Binds to the Fc region of immunoglobulins gamma. Low affinity receptor. By binding to IgG it initiates cellular responses against pathogens and soluble antigens. Promotes phagocytosis of opsonized antigens. Reported localization: Cell membrane. Expression/tissue context: Found on monocytes, neutrophils and eosinophil platelets.
Research relevance and current trends
- Hematopoietic Progenitors: Researchers commonly examine how FCGR2A (Keratin, type I cytoskeletal 14) relates to this theme using model systems and orthogonal readouts.
- Immunoglobulins: Researchers commonly examine how FCGR2A (Keratin, type I cytoskeletal 14) relates to this theme using model systems and orthogonal readouts.
- Immunology: Researchers commonly examine how FCGR2A (Keratin, type I cytoskeletal 14) relates to this theme using model systems and orthogonal readouts.
Common research applications
- Western blotting: compare relative FCGR2A (Keratin, type I cytoskeletal 14) levels across conditions; band patterns may reflect isoforms and processing.
- IHC/IHC-F: assess spatial distribution of FCGR2A (Keratin, type I cytoskeletal 14) across tissue regions and cell types using matched controls.
- ELISA-compatible use: when applicable, interpret signal as relative abundance across sample sets with consistent handling and dilution strategy.
Notes for experimental interpretation
- Specificity notes: No cross reactivity with other proteins.
- Cross-reactivity: No cross-reactivity with other proteins.
- Isoforms and PTMs: Apparent size and signal patterns can differ across splice isoforms, proteolytic processing, and post-translational modifications.
- Controls: Include an isotype control (as relevant), no-primary control for imaging, and orthogonal validation such as KD/KO samples when available.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
