| Field | Specification |
|---|---|
| Target | |
| Alternative names | Amyloid beta precursor protein binding family B member 1; Amyloid-beta A4 precursor protein-binding family B member 1; Protein Fe65; APBB1; FE65; RIR |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human FE65 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 23A71 is a rabbit monoclonal antibody that recognizes amyloid beta precursor protein binding family B member 1 (gene APBB1) in human samples and is validated for Western blot, IHC and ICC/IF. The predicted molecular weight is 77.2 kDa, and the supplier reports an observed band at about 100 kDa.
APBB1 (Fe65) is an adaptor protein that binds the cytoplasmic domain of the amyloid precursor protein (APP). With the APP intracellular domain released by γ-secretase it forms a nuclear complex that regulates transcription, and it also takes part in the DNA damage response, so it is studied in Alzheimer's disease research.
| Target | Amyloid beta precursor protein binding family B member 1 (gene APBB1; UniProt O00213, human) |
|---|---|
| Clone | 23A71 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 77.2 kDa |
| Observed MW | 100 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
Samples with a confirmed band (WB): SH-SY5Y cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human FE65.
Reactivity Notes
The supplier lists reactivity with human. UniProt places amyloid beta precursor protein binding family B member 1 in the cell membrane, cytoplasm, nucleus and cell projection. Tissue expression noted by UniProt (human): Highly expressed in brain. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
