| Field | Specification |
|---|---|
| Target | |
| Alternative names | Fibroblast growth factor 1;FGF-1;Acidic fibroblast growth factor;aFGF;Endothelial cell growth factor;ECGF;Heparin-binding growth factor 1;HBGF-1;FGF1;FGFA; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E. coli-derived human FGF1 recombinant protein (Position: F16-D155). Human FGF1 shares 96.4% amino acid (aa) sequence identity with both mouse and rat FGF1. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets fibroblast growth factor 1 (gene FGF1) in human, mouse and rat samples and is validated for ELISA, IHC and Western blot. The predicted molecular weight is 17.5 kDa, and the supplier reports an observed band at about 24 kDa.
Fibroblast growth factor 1 (FGF1, acidic FGF) is a heparin-binding growth factor that activates all FGF receptors. It lacks a classical signal peptide but acts as a potent mitogen, and it regulates cell proliferation, survival, migration, differentiation and angiogenesis. FGF1 is studied in tissue repair, development and metabolic regulation.
| Target | Fibroblast growth factor 1 (gene FGF1; UniProt P05230, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3 |
| Calculated MW | 17.5 kDa |
| Observed MW | 24 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| IHC (paraffin sections) | 0.5–1 µg/mL (Human, Mouse, Rat) |
|---|---|
| ELISA | 0.1–0.5 µg/mL |
| Western blot | 0.1–0.5 µg/mL (Rat, Human) |
Samples with a confirmed band (WB): rat cardiac muscle tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): mouse intestine tissue, rat intestine tissue, human placenta tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Immunogen
Recombinant human FGF1 fragment (Phe16–Asp155), expressed in E. coli. Sequence identity with the mouse and rat orthologs: 96.4%.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt describes fibroblast growth factor 1 as secreted and also located in the cytoplasm and nucleus. Tissue expression noted by UniProt (human): Predominantly expressed in kidney and brain. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Yizhou Yao et al. (2020) Loss of AKR1B10 promotes colorectal cancer cells proliferation and migration via regulating FGF1-dependent pathway. Aging-US. 10.18632/aging.103393
dos Santos Julia Matzenbacher et al. (2020) Levels of plasma glycan-binding auto-IgG biomarkers improve the accuracy of prostate cancer diagnosis. Molecular and Cellular Biochemistry. 10.1007/s11010-020-03876-7
