| Field | Specification |
|---|---|
| Target | |
| Alternative names | Fibroblast growth factor 2;FGF-2;Basic fibroblast growth factor;bFGF;Heparin-binding growth factor 2;HBGF-2;FGF2;FGFB; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of human FGF2, different from the related mouse and rat sequences by one amino acid. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes fibroblast growth factor 2 (gene FGF2) in human and rat samples and is validated for Western blot. The predicted molecular weight is 30.8 kDa; the supplier reports observed bands at 18, 20 kDa.
Fibroblast growth factor 2 (FGF2, basic FGF) is a heparin-binding growth factor that activates FGF receptors 1–4. It is a potent mitogen that promotes cell proliferation, survival, migration and angiogenesis, and it is widely used as a supplement to maintain stem cells in culture. FGF2 is studied in development, tissue repair, angiogenesis and cancer.
| Target | Fibroblast growth factor 2 (gene FGF2; UniProt P09038, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 30.8 kDa |
| Observed MW | 18, 20 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human, Rat) |
|---|
Samples with a confirmed band (WB): human HeLa cells, human SKOV3 cells, rat liver tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Synthetic peptide from the C-terminal region of human FGF2. It differs from the mouse and rat sequences at one residue.
Reactivity Notes
The supplier lists reactivity with human and rat. UniProt describes fibroblast growth factor 2 as secreted and also located in the nucleus. Tissue expression noted by UniProt (human): Expressed in granulosa and cumulus cells. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Luo Simin et al. (2022) Next-Generation Sequencing of Circular RNAs Reveals the Molecular Mechanisms of Chondrogenic Differentiation in Human Adipose-derived Stem Cells. Cell Biochemistry and Biophysics. 10.1007/s12013-022-01062-8
Hongliang Zhang et al. (2022) MicroRNA‑146a attenuates isoproterenol‑induced cardiac fibrosis by inhibiting FGF2. Experimental and Therapeutic Medicine. 10.3892/etm.2022.11433
Jingjun Xia et al. (2017) Effects of unfractionated heparin and rivaroxaban on the expression of heparanase and fibroblast growth factor 2 in human osteoblasts. Molecular Medicine Reports. 10.3892/mmr.2017.6570
Chao Hu et al. (2015) Effect of Vacuum-Assisted Closure Combined with Open Bone Grafting to Promote Rabbit Bone Graft Vascularization. Medical Science Monitor. 10.12659/MSM.892939
Gonglin Hou et al. (2014) Changes of ultrastructure and downregulation of heat shock protein 70 and fibroblast growth factor 2 in gastric mucosa of rats with depressive-like behaviour. ACTA Neuropsychiatrica. 10.1111/j.1601-5215.2011.00599.x
