| Field | Specification |
|---|---|
| Target | |
| Alternative names | Fibroblast growth factor 2; FGF-2; Basic fibroblast growth factor; bFGF; Heparin-binding growth factor 2; HBGF-2; FGF2; FGFB |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E. coli-derived human FGF2 recombinant protein (Position: P143-S288). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets fibroblast growth factor 2 (gene FGF2) in human samples and is validated for ELISA, ICC/IF, IHC and Western blot. The predicted molecular weight is 30.8 kDa, and the supplier reports an observed band at about 17–21 kDa.
Fibroblast growth factor 2 (FGF2, basic FGF) is a heparin-binding growth factor that activates FGF receptors 1–4. It is a potent mitogen that promotes cell proliferation, survival, migration and angiogenesis, and it is widely used as a supplement to maintain stem cells in culture. FGF2 is studied in development, tissue repair, angiogenesis and cancer.
| Target | Fibroblast growth factor 2 (gene FGF2; UniProt P09038, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.01 mg NaN3 |
| Calculated MW | 30.8 kDa |
| Observed MW | 17–21 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL |
|---|---|
| IHC (paraffin sections) | 2–5 µg/mL |
| Immunocytochemistry / Immunofluorescence | 5 µg/mL |
| ELISA (capture antibody) | 1–5 µg/mL |
Samples with a confirmed band (WB): human SKOV3 cells, human HeLa cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): human mammary cancer tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Samples with confirmed staining (ICC/IF): A431 cells.
Immunogen
Recombinant human FGF2 fragment (Pro143–Ser288), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human. UniProt describes fibroblast growth factor 2 as secreted and also located in the nucleus. Tissue expression noted by UniProt (human): Expressed in granulosa and cumulus cells. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
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