| Field | Specification |
|---|---|
| Target | |
| Alternative names | Fibroblast growth factor 2; FGF-2; Basic fibroblast growth factor; bFGF; Heparin-binding growth factor 2; HBGF-2; FGF2; FGFB |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence at the N-terminus of human FGF2, which shares 93.3% amino acid (aa) sequence identity with mouse and rat FGF2. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes fibroblast growth factor 2 (gene FGF2) in human, mouse and rat samples and is validated for Western blot, IHC and ICC/IF. The predicted molecular weight is 30.8 kDa, and the supplier reports an observed band at about 18 kDa.
Fibroblast growth factor 2 (FGF2, basic FGF) is a heparin-binding growth factor that activates FGF receptors 1–4. It is a potent mitogen that promotes cell proliferation, survival, migration and angiogenesis, and it is widely used as a supplement to maintain stem cells in culture. FGF2 is studied in development, tissue repair, angiogenesis and cancer.
| Target | Fibroblast growth factor 2 (gene FGF2; UniProt P09038, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 30.8 kDa |
| Observed MW | 18 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human) |
|---|---|
| IHC (paraffin sections) | 2–5 µg/mL (Human, Mouse, Rat) |
| Immunocytochemistry / Immunofluorescence | 5 µg/mL (Human) |
Samples with a confirmed band (WB): human K562 cells, human U-87MG cells, human SK-OV-3 cells, human HeLa cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): human gastric cancer tissue, mouse cerebellum tissue, rat cerebellum tissue, human brain tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Samples with confirmed staining (ICC/IF): SiHa cells.
Immunogen
Synthetic peptide from the N-terminal region of human FGF2. Sequence identity with the mouse and rat orthologs: 93.3%.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt describes fibroblast growth factor 2 as secreted and also located in the nucleus. Tissue expression noted by UniProt (human): Expressed in granulosa and cumulus cells. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Mohammad Jaber MasodKhooy et al. (2023) Combinatorial treatment with Silybum marianum essential oil enhances the therapeutic efficacy of a 5-fluorouracil base therapy for hepatocellular carcinoma. Phytotherapy Research. 10.1002/ptr.7716
Xiaoying Kong et al. (2019) Biomimetic hydrogel for rapid and scar-free healing of skin wounds inspired by the healing process of oral mucosa. Acta Biomaterialia. 10.1016/j.actbio.2019.10.011
Wang Hui et al. (2025) Roquin-1 interaction with Regnase-1 inhibits the progression of rheumatoid arthritis via suppressing FGF2 expression and NF-κB pathway. Inflammation Research. 10.1007/s00011-025-02012-9
Bisheng Zhou et al. (2013) MicroRNA-503 targets FGF2 and VEGFA and inhibits tumor angiogenesis and growth. Cancer Letters. 10.1016/j.canlet.2013.01.028
Shuo Tian et al. (2018) Effect of stachydrine hydrochloride to the prostate hyperplasia model in mice. Saudi Journal of Biological Sciences. 10.1016/j.sjbs.2018.12.012
Xiaowen Jiang et al. (2015) Osteogenesis and mineralization in a rabbit mandibular distraction osteogenesis model is promoted by the human LMP-1 gene. Journal of Orthopaedic Research. 10.1002/jor.22811
Nana Liu et al. (2018) The Antimicrobial Peptide Nal-P-113 Exerts a Reparative Effect by Promoting Cell Proliferation, Migration, and Cell Cycle Progression. Biomed Research International. 10.1155/2018/7349351
Zheng Ma et al. (2017) MiR-129-5p inhibits non-small cell lung cancer cell stemness and chemoresistance through targeting DLK1. Biochemical and Biophysical Research Communications. 10.1016/j.bbrc.2017.06.041
Ying Liu et al. (2006) Application of bFGF and BDNF to Improve Angiogenesis and Cardiac Function. Journal of Surgical Research. 10.1016/j.jss.2006.04.034
Changmin Hu et al. (2009) Basic fibroblast growth factor stimulates epithelial cell growth and epithelial wound healing in canine corneas. Veterinary Ophthalmology. 10.1111/j.1463-5224.2009.00696.x
