| Field | Specification |
|---|---|
| Target | |
| Alternative names | Fibroblast growth factor 23; FGF-23; Fgf23 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E. coli-derived rat FGF23 recombinant protein (Position: Y25-V251). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets fibroblast growth factor 23 (gene Fgf23) in mouse and rat samples and is validated for ELISA and Western blot. The predicted molecular weight is 27.9 kDa, and the supplier reports an observed band at about 35 kDa.
Fibroblast growth factor 23 (FGF23) is a hormone made mainly by osteocytes in bone that controls phosphate and vitamin D balance. Acting on the kidney together with the coreceptor Klotho, it reduces phosphate reabsorption and lowers active vitamin D production. FGF23 is studied in phosphate homeostasis, chronic kidney disease and inherited forms of hypophosphatemia.
| Target | Fibroblast growth factor 23 (gene Fgf23; UniProt Q8VI82, rat) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3 |
| Calculated MW | 27.9 kDa |
| Observed MW | 35 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL |
|---|---|
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): rat testis tissue, rat PC-12 cells, mouse testis tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 50 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:10000, ECL detection.
Immunogen
Recombinant rat FGF23 fragment (Tyr25–Val251), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with mouse and rat. UniProt describes fibroblast growth factor 23 as a secreted protein. Tissue expression noted by UniProt (rat): Expressed in the parathyroid. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Kai‑Bao Ji et al. (2017) MicroRNA-296 mediated corneal neovascularization in an animal model of corneal burns after alkali exposures. Experimental and Therapeutic Medicine. 10.3892/etm.2017.5408
