| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Fibroblast growth factor 8; FGF-8; Androgen-induced growth factor; AIGF; Heparin-binding growth factor 8; HBGF-8; FGF8; AIGF; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | E. coli-derived human FGF8 recombinant protein (Position: Q23-R233). |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
This antibody is intended for detection of FGF8 (Protein argonaute-1) in biological samples using common immunoassay formats. It is typically selected based on target identity, species reactivity, clonality/clone information, and detection modality.
Vendor notes: Boster Bio Anti-FGF8 Antibody Picoband® catalog # A00841. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Key elements and design rationale
- Antibody format: Rabbit Polyclonal Rabbit IgG
- Immunogen / epitope context: E. coli-derived human FGF8 recombinant protein (Position: Q23-R233). (reported region: Q23-R233).
- Molecular weight context: reported MW: 26 kDa; calculated MW: 55914 MW
- Reactivity: Human,Mouse,Rat
- Applications: ELISA, WB
As a polyclonal antibody, the reagent recognizes multiple epitopes on the target, which can improve detection robustness but may increase sensitivity to sample-dependent epitope changes.
Biological background
Protein argonaute-1; fibroblast growth factor 8. Fibroblast growth factor 8 (androgen-induced), also known as FGF8 or AIGF, is a human gene which maps to 10q24. The protein encoded by this gene is secreted proteins that interact with FGF tyrosine kinase receptors to mediate growth and development. This protein is known to be a factor that supports androgen and anchorage independent growth of mammary tumor cells. Overexpression of this gene has been shown to increase tumor growth and angiogensis. The temporal and spatial patterns of this gene expression suggest that FGF8 is involved in gastrulation, regionalization of the brain, and organogenesis of the limb and face as an embryonic epithelial factor. The adult expression of FGF8 is restricted to gonads, including testes and ovaries. FGF8 stimulated growth of human prostate carcinoma cells and mouse fibroblasts and mammary carcinoma cells in a dose-dependent manner. It also may play an important role in growth and patterning of limbs, face, and central nervous system. FGF8 is expressed in increased levels in breast cancer and in lactating human breast; it was also detected in human milk. A survey of other normal tissues showed that FGF8 is expressed in the proliferative cells of the skin and epithelial cells in colon, ovary, fallopian tube, and uterus. Functional note: Plays an important role in the regulation of embryonic development, cell proliferation, cell differentiation and cell migration. Required for normal brain, eye, ear and limb development during embryogenesis. Required for normal development of the gonadotropin-releasing hormone (GnRH) neuronal system (PubMed:16384934, PubMed:16597617, PubMed:8663044). Plays a role in neurite outgrowth in hippocampal cells (PubMed:21576111). Reported localization: Secreted. Expression/tissue context: Widely expressed.
Research relevance and current trends
- Angiogenesis: Researchers commonly examine how FGF8 (Protein argonaute-1) relates to this theme using model systems and orthogonal readouts.
- Cancer: Researchers commonly examine how FGF8 (Protein argonaute-1) relates to this theme using model systems and orthogonal readouts.
- Cardiovascular: Researchers commonly examine how FGF8 (Protein argonaute-1) relates to this theme using model systems and orthogonal readouts.
Common research applications
- Western blotting: compare relative FGF8 (Protein argonaute-1) levels across conditions; band patterns may reflect isoforms and processing.
- ELISA-compatible use: when applicable, interpret signal as relative abundance across sample sets with consistent handling and dilution strategy.
Notes for experimental interpretation
- Specificity notes: No cross reactivity with other proteins.
- Cross-reactivity: No cross-reactivity with other proteins.
- Isoforms and PTMs: Apparent size and signal patterns can differ across splice isoforms, proteolytic processing, and post-translational modifications.
- Controls: Include an isotype control (as relevant), no-primary control for imaging, and orthogonal validation such as KD/KO samples when available.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
