| Field | Specification |
|---|---|
| Target | |
| Alternative names | Fibroblast growth factor receptor 1;FGFR-1;2.7.10.1;Basic fibroblast growth factor receptor 1;BFGFR;bFGF-R-1;Fms-like tyrosine kinase 2;FLT-2;N-sam;Proto-oncogene c-Fgr;CD331;FGFR1;BFGFR, CEK, FGFBR, FLG, FLT2, HBGFR; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of human FGFR1, identical to the related mouse and rat sequences. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes fibroblast growth factor receptor 1 (gene FGFR1) in human samples and is validated for Western blot. The predicted molecular weight is 91.9 kDa, and the supplier reports an observed band at about 100 kDa.
Fibroblast growth factor receptor 1 (FGFR1) is a receptor tyrosine kinase that binds FGFs and activates MAPK, PI3K–AKT and PLCγ signaling. It controls cell proliferation, differentiation and migration and is essential for mesoderm formation, skeletal development and GnRH neuron development. FGFR1 amplifications and fusions occur in several cancers.
| Target | Fibroblast growth factor receptor 1 (gene FGFR1; UniProt P11362, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Lyophilized |
| Formulation | Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3; plus stabilizers |
| Calculated MW | 91.9 kDa |
| Observed MW | 100 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human) |
|---|
Samples with a confirmed band (WB): HepG2 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 50 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:10000, ECL detection.
Immunogen
Synthetic peptide from the C-terminal region of human FGFR1. The peptide sequence is conserved in mouse and rat.
Reactivity Notes
The supplier lists reactivity with human. UniProt places fibroblast growth factor receptor 1 in the cell membrane, nucleus, cytoplasm and cytoplasmic vesicle. Tissue expression noted by UniProt (human): Detected in astrocytoma, neuroblastoma and adrenal cortex cell lines. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Yinxian Chen et al. (2020) Circ_0000885 Enhances Osteosarcoma Progression by Increasing FGFR1 Expression via Sponging MiR-1294. Cancer Management and Research. 10.2147/CMAR.S244382
Li Aiping et al. (2021) Expression of fibroblast growth factor 9 and its receptors in the dentate gyrus of hippocampus in poststroke depression rats. Neuroreport. 10.1097/WNR.0000000000001591
