| Field | Specification |
|---|---|
| Target | |
| Alternative names | Fibroblast growth factor receptor 3;FGFR-3;2.7.10.1;CD333;FGFR3;JTK4; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human FGFR3 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone AACG-6 is a rabbit monoclonal antibody that recognizes fibroblast growth factor receptor 3 (gene FGFR3) in human samples and is validated for Western blot, IHC, ICC/IF and flow cytometry. The predicted molecular weight is 87.7 kDa, and the supplier reports an observed band at about 125 kDa.
Fibroblast growth factor receptor 3 (FGFR3) is a receptor tyrosine kinase that responds to FGFs and acts as a brake on chondrocyte proliferation in growing bone. Activating FGFR3 mutations cause achondroplasia and related skeletal dysplasias, and FGFR3 alterations are frequent in bladder cancer, so it is studied in bone growth and oncology.
| Target | Fibroblast growth factor receptor 3 (gene FGFR3; UniProt P22607, human) |
|---|---|
| Clone | AACG-6 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 87.7 kDa |
| Observed MW | 125 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:20–1:100 |
| Immunocytochemistry / Immunofluorescence | 1:50~1:200 FC 1:50 |
| Flow cytometry | Not specified by the supplier |
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:500 overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Immunogen
Synthetic peptide based on the sequence of human FGFR3.
Reactivity Notes
The supplier lists reactivity with human. Tissue expression noted by UniProt (human): Expressed in brain, kidney and testis.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Xi-Feng Fu et al. (2021) MicroRNA‑203‑3p inhibits the proliferation, invasion and migration of pancreatic cancer cells by downregulating fibroblast growth factor 2. Oncology Letters. 10.3892/ol.2021.12887
