| Field | Specification |
|---|---|
| Target | |
| Alternative names | Fibroblast growth factor receptor 4;FGFR-4;2.7.10.1;CD334;FGFR4;JTK2, TKF; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human FGFR4 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone ABHA-6 is a rabbit monoclonal antibody that recognizes fibroblast growth factor receptor 4 (gene FGFR4) in human, mouse and rat samples and is validated for Western blot. The predicted molecular weight is 88.0 kDa, and the supplier reports an observed band at about 88 kDa.
Fibroblast growth factor receptor 4 (FGFR4) is a receptor tyrosine kinase that, together with the coreceptor β-Klotho, mediates FGF19 signaling in the liver. It regulates bile acid synthesis and aspects of lipid and glucose metabolism, and it also contributes to muscle development. FGFR4 is studied in metabolism and in liver cancer.
| Target | Fibroblast growth factor receptor 4 (gene FGFR4; UniProt P22455, human) |
|---|---|
| Clone | ABHA-6 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 88.0 kDa |
| Observed MW | 88 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|
Samples with a confirmed band (WB): human MCF-7 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:500 overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Synthetic peptide based on the sequence of human FGFR4.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places fibroblast growth factor receptor 4 in the cell membrane, endosome and endoplasmic reticulum. Tissue expression noted by UniProt (human): Expressed in gastrointestinal epithelial cells, pancreas, and gastric and pancreatic cancer cell lines. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Xiumei Yan et al. (2022) The water extract of Radix scutellariae, its total flavonoids and baicalin inhibited CYP7A1 expression, improved bile acid, and glycolipid metabolism in T2DM mice. Journal of Ethnopharmacology. 10.1016/j.jep.2022.115238
Hailong Li et al. (2025) Amorphophallus konjac via Gut Microbiota-FXR/FGF15 Axis Changed Bile Acid Metabolism in Hyperlipidemia Mice. Journal of Food Biochemistry. 10.1155/jfbc/8852564
Lu Xu et al. (2023) Zhuyu Pill Alleviates Nonalcoholic Fatty Liver Disease by Regulating Bile Acid Metabolism through the Gut–Liver Axis. ACS Omega. 10.1021/acsomega.3c01955
