| Field | Specification |
|---|---|
| Target | |
| Alternative names | Fibrinogen beta chain; FGB |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence in the middle region of human FGB, different from the related mouse sequence by three amino acids, and from the related rat sequence by five amino acids. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 6D12 is a mouse monoclonal antibody that recognizes fibrinogen beta chain (gene FGB) in human samples and is validated for Western blot and IHC. The predicted molecular weight is 55.9 kDa, and the supplier reports an observed band at about 56 kDa.
Fibrinogen beta chain (FGB) is one of the three chains of fibrinogen, the liver-derived plasma protein that is converted by thrombin into fibrin. Fibrin polymers form the structural scaffold of blood clots and support early wound repair. FGB is studied in coagulation, thrombosis and inflammation.
| Target | Fibrinogen beta chain (gene FGB; UniProt P02675, human) |
|---|---|
| Clone | 6D12 |
| Host / Clonality / Isotype | Mouse / Monoclonal / Mouse IgG2b |
| Reactivity | Human |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 55.9 kDa |
| Observed MW | 56 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human) |
|---|---|
| IHC (paraffin sections) | 2–5 µg/mL (Human) |
Samples with a confirmed band (WB): human HepG2 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 50 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:10000, ECL detection.
Samples with confirmed staining (IHC): human liver cancer tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Immunogen
Synthetic peptide from the middle region of human FGB. It differs from the mouse sequence at three residues and from the rat sequence at five residues.
Reactivity Notes
The supplier lists reactivity with human. UniProt describes fibrinogen beta chain as a secreted protein. Tissue expression noted by UniProt (human): Detected in blood plasma (at protein level). The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Gong Huimin et al. (2022) Pleural thickening induced by Glaesserella parasuis infection was linked to increased collagen and elastin. Frontiers in Cellular and Infection Microbiology. 10.3389/fcimb.2022.952377
Changzhi Zhao et al. (2025) Histological analysis of thrombus composition in acute ischemic stroke with large vessel occlusion: impact of r-tPA treatment. Frontiers in Neuroscience. 10.3389/fnins.2025.1707934
Bin Ma et al. (2025) Glaesserella parasuis induces tissue transglutaminase-mediated fibrinogen crosslinking through NF-κB activation. Veterinary Microbiology. 10.1016/j.vetmic.2025.110418
