Anti-Fibulin 5 Rabbit Monoclonal Antibody

SKU:BHA21009240
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Suppliers
Boster Bio
Boster Bio
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Overview
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Anti-FBLN5 antibody (Rabbit monoclonal, clone 17F39, IgG). Validated for Western blot. Reactivity: Human, Mouse. Recognizes fibulin-5. Observed band in Western blot: about 50 kDa. Supplied in liquid form, ready for use in standard immunodetection workflows.
Target FBLN5
clone number 17F39
Host Rabbit
Reactivity Human,Mouse
Isotype IgG
Application(s) WB
Options selector
Catalog no. Size Conjugation
M02317-1 100 uL/vial
Available Options

Select the variant that best fits your experiment. Availability and lead time may vary by option.

  • Options:
    • Size: 100 uL/vial; Conjugation: Unconjugated
      Form: Liquid
      Storage: Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.
      Applications: WB
      Application details: WB 1:500-2000<br>
      Contents: Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
  • Lead time: typically ships in ~2-3 business days; timing may vary by selected option.
  • Storage: Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.
  • Shipping: cold-chain shipment (typically with ice packs).
  • Upon receipt: store at the recommended temperature as soon as possible.
  • Sales terms and conditions: Please review prior to ordering.
Field Specification
Target FBLN5
Alternative names Fibulin-5; FIBL-5; Developmental arteries and neural crest EGF-like protein; Dance; Urine p50 protein; UP50; FBLN5
Gene ID 10516
UniProt # Q9UBX5
Host Rabbit
Clone 17F39
Clonality
  • Monoclonal
Isotype
  • IgG
Reactivity
  • Human
  • Mouse
Applications
  • Western Blot
Immunogen A synthesized peptide derived from human Fibulin 5
Molecular weight 50.2 kDa
Purification Affinity-chromatography
Reconstitution Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Cellular localization Secreted. Secreted, extracellular space, extracellular matrix.
Concentration 0.5mg/ml
Form Liquid
Storage Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.
Catalog no. (Mfr.) M02317-1
Main SKU BHA21009240
Rabbit Monoclonal

Product Overview

Clone 17F39 is a rabbit monoclonal antibody that recognizes fibulin-5 (gene FBLN5) in human and mouse samples and is validated for Western blot. The predicted molecular weight is 50.2 kDa, and the supplier reports an observed band at about 50 kDa.

Fibulin-5 is a secreted matrix protein needed for the formation of elastic fibers. It helps link elastin to microfibrils and organizes elastic fibers in skin, lung and blood vessels, and it can also bind integrins on endothelial cells. Fibulin-5 is studied in connective tissue biology, vascular elasticity and cutis laxa.

Target Fibulin-5 (gene FBLN5; UniProt Q9UBX5, human)
Clone 17F39
Host / Clonality / Isotype Rabbit / Monoclonal / IgG
Reactivity Human, Mouse
Form Liquid
Formulation PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers
Calculated MW 50.2 kDa
Observed MW 50 kDa
Storage Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles.

Validated Applications

Western blot 1:500–1:2000

Samples with a confirmed band (WB): mouse heart.

Recommended loading (WB): 20–40 µg of total protein per lane.

Immunogen

Synthetic peptide based on the sequence of human fibulin 5.

Reactivity Notes

The supplier lists reactivity with human and mouse. UniProt describes fibulin-5 as a secreted protein. Tissue expression noted by UniProt (human): Expressed in skin fibroblasts (at protein level). The samples tested by the supplier (listed above) are a practical starting point for positive controls.

Safety

Handling: the formulation contains sodium azide as a preservative. Wear gloves, avoid contact with skin and eyes, and dispose of waste according to local regulations. For research use only; not for diagnostic or therapeutic procedures.
Q.Which applications is this antibody validated for, and at what dilution?
A.Validated applications with suggested starting dilutions: Western blot 1:500–1:2000. Optimal dilutions should be determined by each laboratory.
Q.Which species does this antibody react with?
A.Validated reactivity: Human, Mouse. Other species are not listed by the supplier.
Q.What band size should be expected in Western blot?
A.Reported by the supplier: calculated molecular weight 50.2 kDa; observed band at about 50 kDa.
Q.What immunogen was used to raise this antibody?
A.Synthetic peptide based on the sequence of human fibulin 5.
Q.How should this antibody be stored?
A.Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles.

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.

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Experience the power of Celltrypse™, c-LEcta's innovative enzyme solution for gentle and efficient cell dissociation. Request your free sample and discover a superior alternative for your cell culture workflows.

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