Anti-FKBP52 Rabbit Monoclonal Antibody

Overview

This product is an anti-FKBP4 antibody for target detection and characterization. Key identifiers include host species: Rabbit; Monoclonal; clone 20F13; isotype IgG; reactivity: Human,Mouse,Rat. Reported application contexts include WB, IHC, ICC, IF, Flow (as provided in the source record). Boster Bio Anti-FKBP52 Rabbit Monoclonal Antibody catalog # M02165-2. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.

Key elements and design rationale

• Target: FKBP4 (Dual specificity protein phosphatase 6).
• Antibody format: Monoclonal; clone 20F13; isotype IgG.
• Host: Rabbit.
• Species reactivity: Human,Mouse,Rat (confirm in your model system with appropriate controls).

This description is intended to help interpret the antibody design and the biological context of the target using the fields provided in the catalog record, alongside general experimental considerations.

Biological background

FKBP4 (protein: Lysosome-associated membrane glycoprotein 2 (Lamp2)) is a commonly studied target in molecular and cellular biology. Functional context (as provided): Inactivates MAP kinases. Has a specificity for the ERK family (PubMed:9858808). Plays an important role in alleviating chronic postoperative pain. Necessary for the normal dephosphorylation of the long-lasting phosphorylated forms of spinal MAPK1/3 and MAP kinase p38 induced by peripheral surgery, which drives the resolution of acute postoperative allodynia (By similarity). . Reported cellular localization context: Cytoplasm . Tissue expression notes (as provided): Highly expressed in cerebellum, but also found in frontal cortex, hippocampus and basal ganglia.

Research relevance and current trends

• Research context keywords from the source record include: Neural Signal Transduction,Neurology Process,Neuroscience.
• Current studies often focus on connecting target abundance/localization to pathway perturbations across models, tissues, and cell states.
• Quantitative and multiplexed assays (e.g., imaging + immunoblot panels) are commonly used to compare phenotypes across conditions and time-courses.

Common research applications

• Western blotting (WB): assess relative target abundance across samples, treatments, or time-points.
• Immunohistochemistry (IHC): evaluate spatial distribution of target-positive staining in tissue architecture.
• Immunofluorescence/ICC (IF/ICC): visualize subcellular localization patterns and cell-to-cell heterogeneity.
• Flow cytometry: quantify target-positive populations and compare shifts in marker distributions.

Workflow ideas (metafield): Validate FKBP4 antibody specificity using KO/KD control samples (WB/IF/IHC as appropriate), Detect FKBP4 expression by Western blot in cell or tissue lysates, Detect FKBP4 in FFPE tissue sections by immunohistochemistry, Localize FKBP4 by immunofluorescence/immunocytochemistry in cultured cells, Quantify FKBP4-positive cells by flow cytometry in single-cell suspensions

Notes for experimental interpretation

• Consider isoforms and post-translational modifications (PTMs) that may shift apparent molecular weight or epitope accessibility.
• Apparent molecular weight may vary by sample type and processing (observed MW: 52 kDa; calculated MW: 42320 MW).
• Control concepts: include appropriate negative controls (e.g., isotype, KO/KD samples) and orthogonal validation when feasible.

Additional product details (from the source record)

• Molecular weight (observed): 52 kDa
• Cellular localization (provided): Cytoplasm .
• Tissue details (provided): Highly expressed in cerebellum, but also found in frontal cortex, hippocampus and basal ganglia.

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.