| Field | Specification |
|---|---|
| Target | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | Synthetic Peptide of Flag-Tag |
| Conjugate | |
| Purity | |
| Purification | |
| Formulation | |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This monoclonal antibody (clone 12M11), raised in Mouse, recognizes Flag-Tag. It is validated for Western Blot, ELISA, Immunoprecipitation, Immunofluorescence.
Validated Applications
| Applications | Western Blot, ELISA, Immunoprecipitation, Immunofluorescence |
|---|---|
| WB | 1:10000-50000 |
| ELISA | 1:20000-50000 |
| IP | 1:50-1:200 |
| IF | 1:1000 |
Optimal dilutions should be determined by the end user for each application and sample type.
Immunogen
Synthetic Peptide of Flag-Tag
Specifications
| Host | Mouse |
|---|---|
| Clonality | Monoclonal |
| Clone | 12M11 |
| Isotype | IgG1 |
| Conjugate | Unconjugated |
| Reactivity | Species independent |
| Immunogen | Synthetic Peptide of Flag-Tag |
| Specificity | This recombinant antibody can highly specifically recognize the Flag tags of C-terminal and N-terminal of recombinant protein. Variable region gene of this antibody comes from the mouse gene library i |
| Purification | Protein A purified. |
| Purity | >95% SDS-PAGE |
| Formulation | Liquid in PBS pH7.4, containing 0.5% BSA, 0.02% Sodium Azide and 50% Glycerol. |
| Storage | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Reactivity Notes
Validated reactivity: Species independent. For other species, epitope conservation may allow cross-reactivity; confirm with appropriate controls.
Safety & Handling
This product is for research use only (RUO) and is not intended for diagnostic or therapeutic use. The formulation contains sodium azide; handle with standard laboratory precautions.
Related Products
Explore related antibodies and reagents for Flag-Tag research from St John's Laboratory Ltd, or contact us for alternative formats.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
