| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Flag tag, Flag tag (DYKDDDDK-tag), Flag-tag, Flag peptide (DYKDDDDK) |
| Clonality | |
| Form | Liquid |
| Host | |
| Isotype | |
| Product Type | |
| Storage |
Overview
Anti-Flag Tag (DYKDDDDK) Antibody (2H8) is intended for detection of Flag Tag. Commonly used in CHIP, IF, IP, WB applications. Typical workflows include Select staining controls in cells/tissues (isotype/KD/KO), Validate antibody specificity by WB with KD/KO control lysates, Detect target localization by IF/ICC in cultured cells, Enrich target by IP/Co-IP from cell lysates for downstream analysis, Map DNA binding by ChIP-qPCR in crosslinked chromatin, Optimize antibody dilution for staining in cells/tissues using a reference sample. Relevant research areas: Molecular & Cellular Biology.
Also known as: Flag peptide, Flag-tag.
Reagent details: Host: Mouse; Clonality: Monoclonal; Isotype: IgG2a, kappa; Purification: Protein A/G purified from cell culture supernatant.; Format: Liquid.
Target biology
Flag Tag (Flag peptide) is the antigen described for this antibody and is commonly evaluated for expression and localization in relevant model systems. It is frequently investigated in contexts related to Molecular & Cellular Biology. Common alternative names and symbols used in the literature include: Flag-tag. Based on the annotated name/class, the antigen is most often associated with context-dependent localization, though this can vary by model system.
How this reagent helps
This antibody enables sensitive detection of the intended antigen in common research assays. For best performance, interpret signal in the context of appropriate controls and orthogonal validation when available.
Recommended experimental notes
- Titrate the antibody/reagent to optimize signal-to-noise for your specific sample type and detection system.
- Include appropriate controls (e.g., unstained, secondary-only where relevant, and biological positives/negatives).
- For IF/ICC, test fixation (PFA vs methanol) and permeabilization conditions to preserve epitope accessibility while minimizing background.
- For Western blotting, include fresh protease inhibitors (and phosphatase inhibitors for PTM targets) and verify the dominant band at the expected size; additional bands may reflect isoforms, processing, or cross-reactivity.
- For immunoprecipitation, use gentle lysis conditions, maintain cold temperatures, and validate enrichment relative to an isotype control.
Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term. 0.01M PBS, pH 7.4.
Additional notes
For research use only.
For research use only. Not for diagnostic procedures.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
