Anti-FLI1 Picoband® Antibody

Overview

Anti-FLI1 Picoband® Antibody is an antibody for FLI1 detection raised in Rabbit (Polyclonal, Rabbit IgG), with reported reactivity: Human,Mouse,Rat. Commonly used in WB, IHC, IF, ICC, Flow Cytometry, ELISA workflows.

Key elements and design rationale

• Target: FLI1 (Fli-1 proto-oncogene, ETS transcription factor); UniProt: Q01543; NCBI Gene: 2313
• Antibody format: Rabbit, Polyclonal, Rabbit IgG
• Molecular weight: 54 kDa, calculated 62378 MW
• Applications: WB, IHC, IF, ICC, Flow Cytometry, ELISA

Vendor description (summary): Boster Bio Anti-FLI1 Picoband® Antibody catalog # A00399.

Biological background

Biological context: Sequence-specific transcriptional activator. Recognizes the DNA sequence 5'-C[CA]GGAAGT-3'.

Expression and localization notes: cellular localization: Nucleus., tissue context: Expressed in most tissues tested, including skin, lung, kidney, heart, testis and stomach..

Common research applications

• Western blotting (WB): Compare FLI1 levels across samples and conditions using appropriate loading and biological controls.
• Immunohistochemistry (IHC): Evaluate spatial distribution of FLI1 in tissue sections, considering fixation and antigen retrieval effects.
• Immunofluorescence / ICC: Assess subcellular localization patterns and co-localization with compartment markers in cultured cells.
• Flow cytometry: Quantify FLI1-positive populations in single-cell suspensions with appropriate gating and controls.
• ELISA: Use antibody-based detection formats to assess antigen presence or binding in plate-based assays.

Notes for experimental interpretation

• Account for isoforms, post-translational modifications, and sample-specific processing that can shift apparent molecular weight or epitope accessibility.
• Use positive/negative biological controls where possible (e.g., known-expressing cells/tissues, knockdown/knockout models) and include appropriate secondary-only/isotype controls for imaging workflows.

Additional product notes (from provided fields)

• Specificity: No cross reactivity with other proteins.
• Background: Friend leukemia integration 1 transcription factor (FLI1), also known as transcription factor ERGB, is a protein that in humans is encoded by the FLI1 gene, which is a proto-oncogene. It is mapped to 11q24.3. This gene encodes a transcription factor containing an ETS DNA-binding domain. The gene can undergo a t (11;22) (q24;q12) translocation with the Ewing sarcoma gene on chromosome 22, which results in a fusion gene that is present in the majority of Ewing sarcoma cases. An acute lymphoblastic leukemia-associated t (4;11) (q21;q23) translocation involving this gene has also been identified. Alternative splicing results in multiple transcript variants.
• Cross reactivity: No cross-reactivity with other proteins.
• Cellular localization: Nucleus.
• Tissue details: Expressed in most tissues tested, including skin, lung, kidney, heart, testis and stomach.
• Research category: Class I,Cytoskeleton,Cytoskeleton/ECM,Intermediate Filaments,Signal Transduction

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.