| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Friend leukemia integration 1 transcription factor;Proto-oncogene Fli-1;Transcription factor ERGB;FLI1; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Gene ID | |
| Host | |
| Immunogen | A synthesized peptide derived from human FLI1 |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
Anti-FLI1 Rabbit Monoclonal Antibody is an antibody targeting FLI1. Common applications include WB, IHC. Key specifications include host: Rabbit; clonality: Monoclonal; clone: Clone: ECC-6; isotype: Rabbit IgG; reactivity: Human,Mouse; observed MW: 43 kDa; calculated MW: 50982 MW.
Boster Bio Anti-FLI1 Rabbit Monoclonal Antibody catalog # M00399-1. Tested in WB, IHC applications. This antibody reacts with Human, Mouse.
Key elements and design rationale
- Target: FLI1 — Friend leukemia integration 1 transcription factor
- Antibody format: Host: Rabbit; Clonality: Monoclonal; Clone: Clone: ECC-6; Isotype: Rabbit IgG
- Species reactivity: Human,Mouse
- Molecular weight guidance: Observed: 43 kDa; Calculated: 50982 MW
Biological background
Protein function (datasheet): Sequence-specific transcriptional activator. Recognizes the DNA sequence 5'-C[CA]GGAAGT-3'.
Cellular localization (datasheet): Nucleus.
Tissue details (datasheet): Released primarily from activated T lymphocytes.
Research relevance and current trends
- Commonly studied in contexts related to Apoptosis,Cancer,Cancer Susceptibility,Cell Biology,Cell Cycle,Cell Death,2339,Domain Families,Epigenetics and Nuclear Signaling,Metabolism,Metabolism Processes,Nuclear,Nucleus,Pathways and Processes,Proto-Oncogenes,Transcription.
- Supports comparative expression analysis across conditions, genotypes, or treatments when paired with appropriate controls.
- Useful for confirming target presence and subcellular distribution using orthogonal readouts (e.g., microscopy vs. immunoblotting).
Common research applications
- Western blot (WB): Compare relative target abundance and apparent size/isoforms across samples; interpret bands in light of expected MW and potential PTMs.
- Immunohistochemistry (IHC): Assess tissue distribution and cell-type patterns; interpret staining with appropriate negative controls and antigen context.
Notes for experimental interpretation
- Consider isoforms, post-translational modifications, and processing that can shift apparent molecular weight or localization.
- Use appropriate positive and negative controls (e.g., KO/KD, blocking peptide, or isotype controls) to support specificity interpretation.
As a monoclonal antibody, this reagent is expected to recognize a defined epitope, which can support consistency across lots when epitope accessibility is preserved.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
