| Field | Specification |
|---|---|
| Target | |
| Alternative names | Flotillin-2;Epidermal surface antigen;ESA;Membrane component chromosome 17 surface marker 1;FLOT2;ESA1, M17S1; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human Flotillin 2 recombinant protein (Position: K169-K344). Human Flotillin 2 shares 100% amino acid (aa) sequence identity with both mouse and rat Flotillin 2. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes flotillin-2 (gene FLOT2) in human, mouse and rat samples and is validated for flow cytometry, ICC/IF, IHC and Western blot. The predicted molecular weight is 47.1 kDa, and the supplier reports an observed band at about 47–50 kDa.
Flotillin-2 is a membrane scaffolding protein found in lipid raft microdomains, where it forms hetero-oligomers with flotillin-1. It participates in membrane trafficking, endocytosis and cell adhesion, including in the epidermis. Flotillin-2 is studied in membrane biology and as a marker of exosomes and lipid rafts.
| Target | Flotillin-2 (gene FLOT2; UniProt Q14254, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3; plus stabilizers |
| Calculated MW | 47.1 kDa |
| Observed MW | 47–50 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL |
|---|---|
| IHC (paraffin sections) | 0.5–1 µg/mL |
| IHC (frozen sections) | 0.5–1 µg/mL |
| Immunocytochemistry / Immunofluorescence | 2 µg/mL |
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells |
Samples with a confirmed band (WB): human 293T cells, human HeLa cells, human A549 cells, rat lung tissue, mouse lung tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C.
Samples with confirmed staining (IHC): human mammary cancer tissue, mouse intestine tissue, rat kidney tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Samples with confirmed staining (IHC): rat kidney tissue, rat small intestine tissue, mouse kidney tissue.
Samples with confirmed staining (ICC/IF): A431 cells.
Samples with a confirmed signal (flow cytometry): RH35 cells, U2OS cells.
Immunogen
Recombinant human Flotillin 2 fragment (Lys169–Lys344), expressed in E. coli. Sequence identity with the mouse and rat orthologs: 100%.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places flotillin-2 in the cell membrane, membrane and endosome. Tissue expression noted by UniProt (human): In skin, expressed in epidermis and epidermal appendages but not in dermis. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
