| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Dimethylaniline monooxygenase [N-oxide-forming] 1;1.14.13.8;Dimethylaniline oxidase 1;Fetal hepatic flavin-containing monooxygenase 1;FMO 1;FMO1; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of human FMO1, different from the related mouse sequence by one amino acid, and from the related rat sequence by two amino acids. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
This antibody is intended for detection of FMO1 (Dimethylaniline monooxygenase [N-oxide-forming] 1) in biological samples using common immunoassay formats. It is typically selected based on target identity, species reactivity, clonality/clone information, and detection modality.
Vendor notes: Boster Bio Anti-FMO1 Antibody Picoband® catalog # PB9589. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Key elements and design rationale
- Antibody format: Rabbit Polyclonal Rabbit IgG
- Immunogen / epitope context: A synthetic peptide corresponding to a sequence at the C-terminus of human FMO1, different from the related mouse sequence by one amino acid, and from the related rat sequence by two amino acids.
- Molecular weight context: reported MW: 60 kDa; calculated MW: 60 kDa
- Reactivity: Human,Mouse,Rat
- Applications: WB
As a polyclonal antibody, the reagent recognizes multiple epitopes on the target, which can improve detection robustness but may increase sensitivity to sample-dependent epitope changes.
Biological background
Dimethylaniline monooxygenase [N-oxide-forming] 1; Dimethylaniline monooxygenase [N-oxide-forming] 1. Metabolic N-oxidation of the diet-derived amino-trimethylamine (TMA) is mediated by flavin-containing monooxygenase and is subject to an inherited FMO3 polymorphism in man resulting in a small subpopulation with reduced TMA N-oxidation capacity resulting in fish odor syndrome Trimethylaminuria. Three forms of the enzyme, FMO1 found in fetal liver, FMO2 found in adult liver, and FMO3 are encoded by genes clustered in the 1q23-q25 region. Flavin-containing monooxygenases are NADPH-dependent flavoenzymes that catalyzes the oxidation of soft nucleophilic heteroatom centers in drugs, pesticides, and xenobiotics. Several transcript variants encoding different isoforms have been found for this gene. Functional note: This protein is involved in the oxidative metabolism of a variety of xenobiotics such as drugs and pesticides. Form I catalyzes the N-oxygenation of secondary and tertiary amines. Reported localization: Microsome membrane. Endoplasmic reticulum membrane. Expression/tissue context: Expressed mainly in fetal liver, adult kidney and, to a lesser extent, the intestine.
Research relevance and current trends
- Cancer: Researchers commonly examine how FMO1 (Dimethylaniline monooxygenase [N-oxide-forming] 1) relates to this theme using model systems and orthogonal readouts.
- Drug Metabolism: Researchers commonly examine how FMO1 (Dimethylaniline monooxygenase [N-oxide-forming] 1) relates to this theme using model systems and orthogonal readouts.
- Metabolic Signaling Pathways: Researchers commonly examine how FMO1 (Dimethylaniline monooxygenase [N-oxide-forming] 1) relates to this theme using model systems and orthogonal readouts.
Common research applications
- Western blotting: compare relative FMO1 (Dimethylaniline monooxygenase [N-oxide-forming] 1) levels across conditions; band patterns may reflect isoforms and processing.
Notes for experimental interpretation
- Specificity notes: No cross reactivity with other proteins.
- Cross-reactivity: No cross-reactivity with other proteins
- Family / similarity context: Belongs to the FMO family.
- Isoforms and PTMs: Apparent size and signal patterns can differ across splice isoforms, proteolytic processing, and post-translational modifications.
- Controls: Include an isotype control (as relevant), no-primary control for imaging, and orthogonal validation such as KD/KO samples when available.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
